肝星状细胞通过活化γδ T细胞抑制人HepG2肝癌细胞增殖和侵袭

发布时间：2018-06-06 03:43

本文选题：肝星状细胞 + γδT细胞　；参考：《细胞与分子免疫学杂志》2017年07期

【摘要】：目的研究人肝星状细胞(HSC)作用于γδ T细胞进而对肝癌细胞增殖、侵袭能力产生的影响。方法利用密度梯度离心法及流式细胞术分离获取健康志愿者与肝癌患者的外周血γδ T细胞,使用TranswellTM小室共培养LX-2细胞和γδ T细胞。ELISA检测γδ T细胞上清液中γ干扰素(IFN-γ)含量,CCK-8法检测HepG2的增殖能力,Transwell~(TM)侵袭实验检测HepG2细胞侵袭能力。结果与LX-2细胞共培养后,γδ T细胞分泌IFN-γ量增加,健康志愿者组IFN-γ分泌量增加比肝癌患者组更明显。共培养后γδ T细胞对HepG2细胞增殖、侵袭的抑制能力增强,且健康志愿者组抑制效果增强较肝癌患者组更明显。结论 HSC可以活化γδ T细胞、促进其分泌IFN-γ,进而增强γδ T细胞抑制肝癌细胞增殖和侵袭的能力。
[Abstract]:Objective to study the effect of human hepatic stellate cell (HSC) on the proliferation and invasion of hepatoma cells by 纬未 T cells. Methods 纬未 T cells were isolated from peripheral blood of healthy volunteers and patients with liver cancer by density gradient centrifugation and flow cytometry. TranswellTM co-cultured LX-2 cells and 纬未 T cells. Elisa were used to detect the content of IFN- 纬 in the supernatant of 纬未 T cells. CCK-8 method was used to detect the proliferative ability of HepG2. Results after co-culture with LX-2 cells, the amount of IFN- 纬 secreted by 纬未 T cells increased, and the secretion of IFN- 纬 in healthy volunteers was more obvious than that in patients with liver cancer. After co-culture, 纬未 T cells inhibited the proliferation and invasion of HepG2 cells, and the inhibition effect of 纬未 T cells in healthy volunteers was more obvious than that in patients with liver cancer. Conclusion HSC can activate 纬未 T cells, promote the secretion of IFN- 纬, and then enhance the ability of 纬未 T cells to inhibit the proliferation and invasion of hepatoma cells.
【作者单位】：重庆医科大学附属第一医院肝胆外科;
【基金】：国家临床重点专科建设项目([2012]649号) 国家自然科学基金(81301656) 重庆市渝中区科委基础与前沿研究计划(20140109)
【分类号】：R735.7

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