MIR155HG促进人肺腺癌细胞A549的增殖和迁移侵袭

发布时间：2018-06-06 14:55

  本文选题：长链非编码RNA + MIRHG　； 参考：《重庆医科大学学报》2017年01期

【摘要】：目的:探讨非编码RNA MIR155HG对人肺癌A549细胞增殖、迁移和侵袭能力的影响及机制。方法:过表达或敲减MIR155HG后,MTS检测A549细胞生长的变化,流氏细胞仪检测细胞周期。Transwell迁移和侵袭实验检测过表达或敲减MIR155HG后,A549细胞迁移和侵袭能力的变化。过表达MIR155HG后,定量PCR检测A549细胞中mi R-155-5p的表达。结果:MTS法显示,转染72 h和96 h后,过表达MIR155HG组细胞吸光度(absorbance,A)值分别为(2.47±0.14)和(3.13±0.15),均较对照组[(2.09±0.12)(72 h)和(2.50±0.13)(96 h)]增加(P=0.006,P=0.027);敲减MIR155HG组细胞在48、72和96 h的OD值分别为(1.69±0.15),(1.87±0.09)和(2.24±0.16),较对照组[(2.04±0.06)(48 h),(2.43±016)(72 h)和(2.88±0.11)(96 h)]均降低(P=0.006,P=0.006和P=0.004);敲减MIR155HG组的A549细胞G0/G1期比例为63.93%,与对照组(54.32%)相比,增加了9.61%;迁移实验结果显示,过表达MIR155HG组的穿膜细胞数为(31.67±3.51)个,与对照组(12.67±2.51)相比增加明显(P=0.002)。敲减MIR155HG组的A549细胞的穿膜细胞数为(13.67±3.21)个,与对照组(36.00±4.58)相比明显减少(P=0.002)。侵袭实验结果显示,过表达MIR155HG组细胞穿膜细胞数为(42.33±7.02)个,与对照组(15.67±3.51)相比明显增加(P=0.004)。敲减MIR155HG组穿膜细胞数为(15.00±3.60)个,与对照组(39.00±4.36)相比明显减少(P=0.002)。定量PCR显示,过表达MIR155HG后,mi R-155-5p的表达较对照组增加的倍数为(17.99±2.42)(P=0.000)。结论:MIR155HG能增强A549细胞的增殖、迁移和侵袭能力,其发挥作用的机制可能是通过产生mi R-155-5p来实现。
[Abstract]:Objective: To investigate the effect and mechanism of non coded RNA MIR155HG on proliferation, migration and invasion of human lung cancer A549 cells. Methods: after overexpression or knockout MIR155HG, MTS detected the changes in growth of A549 cells. The flow cytometer detected the expression of.Transwell migration and invasion in the cell cycle, and after the knockout of MIR155HG, and the migration and invasion of A549 cells. After overexpression of MIR155HG, quantitative PCR was used to detect the expression of MI R-155-5p in A549 cells. Results: MTS method showed that after transfection of 72 h and 96 h, the values of overexpressed MIR155HG group cell absorbency (absorbance, A) were (2.47 + 0.14) and (3.13 + 0.15) respectively, which were higher than those of the control group (2.09 + 0.12) (72 h) and (2.50 + 0.13) (96)]. The OD values of MIR155HG group at 48,72 and 96 h were (1.69 + 0.15), (1.87 + 0.09) and (2.24 + 0.16), respectively, compared with the control group [(2.04 + 0.06) (48 h), (2.43 + 016) (72 h) and (P=0.006) (P=0.006 and P=0.004)), and the A549 cell G0/G1 phase ratio of the knockout MIR155HG group was higher than that of the control group. The migration experiment showed that the number of transmembrane cells in the overexpressed MIR155HG group was (31.67 + 3.51), and was significantly increased compared with the control group (12.67 + 2.51). The number of membrane cells in the A549 cells of the MIR155HG group was (13.67 + 3.21), compared with the control group (36 + 4.58). The results of the invasion experiment showed that the overexpression of MIR155H was MIR155H. The number of cell transmembrane cells in the G group was (42.33 + 7.02), compared with the control group (15.67 + 3.51) (P=0.004). The number of membrane cells in the subtraction MIR155HG group was (15 + 3.60), compared with the control group (39 + 4.36). The quantitative PCR showed that the expression of MI R-155-5p was increased by (17.99 + 2) than that of the control group after MIR155HG. .42) (P=0.000). Conclusion: MIR155HG can enhance the proliferation, migration and invasion of A549 cells, and its mechanism may be achieved through the production of MI R-155-5p.
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本文编号：1987040