SB203580与薯蓣皂素对食管癌Eca109细胞调控机制研究

发布时间：2018-06-06 23:27

  本文选题：SB203580 + 薯蓣皂素　； 参考：《江苏大学》2016年硕士论文

【摘要】：目的:进一步探讨薯蓣皂素(Diosgenin,Dio)对人食管癌Eca109细胞的影响的作用机制。方法:体外培养人食管鳞状上皮细胞癌Eca109细胞,用p38特异性抑制剂SB203580(10μg/m L)处理Eca109细胞,Western blot方法用于检测SB203580处理后p38 MAPK蛋白表达水平。SB203580(10μg/m L)和薯蓣皂素(50μg/m L)共处理Eca109细胞,MTT实验用于检测两药共处理后Eca109细胞的增殖情况,Western blot方法检测两药共处理后的Eca109细胞的凋亡情况及磷酸化p38(p-p38MAPK)的蛋白表达水平。结果:在人食管癌Eca109细胞中,p38特异性抑制剂SB203580能有效抑制p38蛋白的表达,Eca109细胞经过50μg/m L的薯蓣皂素和10μg/m L的SB203580处理后,与未使用抑制剂处理组相比,Eca109细胞的增殖进一步被抑制,细胞的凋亡效应进一步增强,p-p38蛋白的表达水平进一步降低。结论:薯蓣皂素调控人食管癌Eca109细胞的增殖,凋亡过程可能是通过p-p38MAPK途径发挥作用的。
[Abstract]:Aim: to investigate the mechanism of diosgenin (Dio) on human esophageal carcinoma Eca109 cells. Methods: human esophageal squamous cell carcinoma (Eca109) cells were cultured in vitro. Treatment of Eca109 cells with p38 specific inhibitor SB203580(10 渭 g / mL Western blot method was used to detect the expression level of p38 MAPK protein. SB20358010 渭 g / mL) and diosgenin 50 渭 g / mL co-treated Eca109 cells were used to detect the proliferation of Eca109 cells after co-treatment with two drugs. The apoptosis of Eca109 cells and the protein expression of phosphorylated p38 MAPK were detected by Western blot. Results: SB203580, a specific inhibitor of p38, could effectively inhibit the expression of p38 protein in human esophageal carcinoma Eca109 cells. After treated with 50 渭 g / mL diosgenin and 10 渭 g / mL SB203580, the proliferation of Eca109 cells was further inhibited. The apoptotic effect further enhanced the expression of p-p38 protein and further decreased the expression level of p-p38 protein. Conclusion: diosgenin regulates the proliferation and apoptosis of human esophageal carcinoma Eca109 cells through p-p38MAPK pathway.
【学位授予单位】：江苏大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R735.1

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