miR-421下调FOXO4增强鼻咽癌细胞CNE-2放疗敏感性

发布时间：2018-06-08 22:16

本文选题：miR- + FOXO　；参考：《基因组学与应用生物学》2017年05期

【摘要】：鼻咽癌(nasopharyngeal carcinoma,NPC)在头颈部肿瘤中位居首位,对人们的身体健康构成严重威胁。放射治疗是目前治疗鼻咽癌的有效手段,但是鼻咽癌组织存在对放射射线不敏感的细胞,这对放疗敏感性提出了更高要求。为探究miR-421下调FOXO4对鼻咽癌细胞CNE-2放疗敏感性的作用,本研究先对miR-421靶基因进行预测,然后考察不同放疗剂量对miR-421和FOXO4 mRNA表达水平的影响,直接证明miR-421及FOXO4与鼻咽癌细胞放疗敏感性有关,最后通过存活曲线、流式细胞仪检测、MTT法,探究了miR-421上调、FOXO4沉默对鼻咽癌细胞CNE-2放疗敏感性的影响。结果发现,FOXO4为miR-421的潜在靶基因,鼻咽癌细胞CNE-2经放射处理后,miR-421的表达水平明显下降,FOXO4的表达水平显著升高;miR-421上调和FOXO4沉默均增强了鼻咽癌细胞放疗的敏感性,降低了癌细胞存活率和克隆形成率,提高了的癌细胞凋亡率。可得知,miR-421通过负调控FOXO4靶基因增强鼻咽癌细胞CNE-2放疗的敏感性,这为进一步深入研究miR-421对其靶基因FOXO4的作用机制打下基础。
[Abstract]:Nasopharyngeal carcinoma (NPC) is the leading tumor in head and neck, which poses a serious threat to people's health. Radiotherapy is an effective method for the treatment of nasopharyngeal carcinoma (NPC) at present, but there are some cells insensitive to radiation in nasopharyngeal carcinoma, which puts forward higher requirements for radiosensitivity. In order to investigate the effect of miR-421 down-regulating the sensitivity of FOXO4 to radiotherapy in nasopharyngeal carcinoma cell line CNE-2, we first predicted the target gene of miR-421, and then investigated the effects of different doses of radiotherapy on the expression of miR-421 and FOXO4 mRNA in nasopharyngeal carcinoma cell line CNE-2. The results showed that miR-421 and FOXO4 were related to the radiosensitivity of nasopharyngeal carcinoma cells. Finally, the effect of miR-421 up-regulating FOXO4 silencing on the radiosensitivity of nasopharyngeal carcinoma cell line CNE-2 was investigated by using survival curve and MTT assay. The results showed that FOXO4 was a potential target gene of miR-421. The expression level of miR-421 in CNE-2 cells was significantly decreased after radiation treatment. Both up-regulation of FOXO4 and silencing of FOXO4 enhanced the sensitivity of NPC cells to radiotherapy. The survival rate and clone formation rate of cancer cells were decreased, and the apoptosis rate of cancer cells was increased. It can be concluded that miR-421 enhances the sensitivity of CNE-2 cells to radiotherapy through negative regulation of FOXO4 target gene, which lays a foundation for further study of the mechanism of miR-421 acting on FOXO4 target gene.
【作者单位】：深圳市宝安区人民医院耳鼻喉科;
【基金】：深圳市宝安区人民医院资助
【分类号】：R739.63

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