TNF-α、IL-1β及IL-6在胃癌诊断中的敏感性和特异性的研究

发布时间：2018-06-09 01:22

本文选题：胃癌 + TNF-α　；参考：《青海大学》2017年硕士论文

【摘要】：目的:探讨炎症因子TNF-α、IL-1β及IL-6联合在胃癌诊断中的临床应用,以提高临床诊断胃癌的灵敏度、诊断率。方法:对青海大学附属医院2015年11月-2016年12月120例经确诊病人的血液标本,将其分成三组,其中,正常对照组、慢性胃炎组和肿瘤组各40例。采用ELISA方法分别对正常对照组、慢性胃炎组和肿瘤组三组研究对象进行血清中TNF-α、IL-1β及IL-6的表达水平检测。比较三种炎症因子在正常对照组、慢性胃炎组和胃癌组的含量与灵敏度,最后利用统计学方法对统计结果进行系统分析。结果:1、采用非参数秩和检验(Kruskal Wallis检验)比较对照组、胃炎组、胃癌组间的TNF-α、IL-1β、IL-6差异,结果显示对照组、胃炎组、胃癌组间的TNF-α、IL-1β、IL-6差异有统计学意义,两两比较显示胃癌组的TNF-α、IL-1β、IL-6均明显高于对照组、胃炎组,对照组和胃炎组的TNF-α、IL-1β、IL-6差异无统计学意义。2、患者血清TNF-α、IL-1β及IL-6含量明显高于正常对照组与慢性胃炎组,有统计学意义(P0.05);3、TNF-α鉴别胃癌的最佳临界值为60.77,灵敏度和特异度分别为92.5%和90%,正确指数为0.825。IL-1β鉴别胃癌的最佳临界值为29.74,灵敏度和特异度分别为77.5%和88.75%,正确指数为0.663。IL-6鉴别胃癌的最佳临界值为5.775,灵敏度和特异度分别为97.5%和85%,正确指数为0.825。TNF-α、IL-1β、IL-6联合诊断鉴别胃癌的最佳临界值为0.37,灵敏度和特异度分别为97.5%和97.5%,正确指数为0.95。结论:三种炎症因子联合诊断胃癌敏感性最高,具有统计学意义(P0.05),能够提高胃癌的诊断率。
[Abstract]:Objective: to explore the clinical application of TNF- 伪 IL-1 尾 and IL-6 in the diagnosis of gastric cancer, in order to improve the sensitivity and diagnostic rate of clinical diagnosis of gastric cancer. Methods: the blood samples of 120 confirmed patients in the affiliated Hospital of Qinghai University from November 2015 to December 2016 were divided into three groups: normal control group, chronic gastritis group and tumor group. The serum levels of TNF- 伪, IL-1 尾 and IL-6 were detected by Elisa in normal control group, chronic gastritis group and tumor group. The content and sensitivity of three kinds of inflammatory factors in normal control group, chronic gastritis group and gastric cancer group were compared. Finally, the statistical results were systematically analyzed by statistical method. Results [WT5HZ] by using nonparametric rank sum test (Kruskal Wallis test), there were significant differences in TNF- 伪 and IL-1 尾 and IL-6 between the control group, gastritis group and gastric cancer group. The results showed that there were significant differences in TNF- 伪 IL-1 尾 and IL-6 between the control group, gastritis group and gastric cancer group. The results showed that the levels of TNF- 伪 IL-1 尾 and IL-6 in gastric cancer group were significantly higher than those in control group. There was no significant difference in TNF- 伪 IL-1 尾 and IL-6 between gastritis group, control group and gastritis group. The serum levels of TNF- 伪 IL-1 尾 and IL-6 in gastric cancer group were significantly higher than those in normal control group and chronic gastritis group. The best critical value for distinguishing gastric cancer is 60.77, the sensitivity and specificity are 92.5% and 90 respectively, the correct index is 0.825.IL-1 尾, the best critical value is 29.74, the sensitivity and specificity are 77.5% and 88.75, respectively. The correct index is 0.663.IL-6. The best critical value for the diagnosis of gastric cancer was 5.775, the sensitivity and specificity were 97.5% and 85%, respectively. The correct index was 0.825.TNF- 伪 IL-1 尾 and IL-6 for the diagnosis of gastric cancer. The best critical value was 0.37, the sensitivity and specificity were 97.5% and 97.5%, respectively, and the correct index was 0.95. Conclusion: the sensitivity of three kinds of inflammatory factors in the diagnosis of gastric cancer is the highest, which has statistical significance (P 0.05) and can improve the diagnosis rate of gastric cancer.
【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.2

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