miR-185通过调控E2F6抑制肺鳞癌细胞增殖和侵袭

发布时间：2018-06-09 20:13

本文选题：肺鳞癌 + miR-　；参考：《第三军医大学学报》2017年02期

【摘要】：目的探讨微小RNA-185(micro RNA-185,miR-185)对肺鳞癌细胞增殖和侵袭能力的作用及其可能的作用机制。方法人肺鳞癌细胞系(NCI-H520、NCI-H226)以及正常肺上皮细胞(BEAS-2B)为实验用细胞;q RT-PCR检测miR-185表达量;miR-NC(对照)和miR-185 mimics(miR-185类似物)利用Turbofect进行转染;Western blot检测E2F6的蛋白表达量;双荧光素酶报告基因检测miR-185与E2F6的靶向调控关系;MTT检测细胞的增殖能力;细胞侵袭试剂盒检测细胞的侵袭能力;构建pc DNA.3.1-E2F6重组载体,并转染肺鳞癌细胞过表达E2F6。结果 miR-185表达量在肺鳞癌细胞中显著降低(P0.01)。miR-185靶向抑制E2F6基因(P0.05)。转染miR-185 mimics显著上调miR-185的细胞含量(P0.01)并抑制E2F6蛋白表达(P0.01),肺鳞癌细胞增殖能力(P0.05或P0.01)和侵袭能力(P0.05)亦显著受抑。转染pc DNA.3.1-E2F6到miR-185含量升高的细胞,E2F6蛋白表达量显著升高(P0.01)且细胞增殖(P0.01)和侵袭能力(P0.05)上升。结论 miR-185可以通过靶向E2F6抑制肺鳞癌细胞增殖和侵袭。
[Abstract]:Objective to investigate the effect of microRNA-185 micro RNA-185 miR-185 on proliferation and invasion of lung squamous cell carcinoma cells and its possible mechanism. Methods the expression of miR-185 in human lung squamous cell carcinoma cell line NCI-H520 (NCI-H226) and normal lung epithelial cell line (BEAS-2B) was detected by RT-PCR. The protein expression of E2F6 was detected by turbofect transfection with Western blot (control) and miR-185 mimicsmiR-185 analogue. Double luciferase reporter gene was used to detect the targeted regulatory relationship between miR-185 and E2F6. MTT assay was used to detect cell proliferation, cell invasion kit was used to detect cell invasion ability, and pc DNA.3.1-E2F6 recombinant vector was constructed and transfected into lung squamous cell carcinoma cells for overexpression of E2F6. Results the expression of miR-185 was significantly decreased in lung squamous cell carcinoma cells. Transfection of miR-185 mimics significantly up-regulated the cell content of miR-185 (P0.01) and inhibited the expression of E2F6 protein (P0.01a), and the proliferation ability of lung squamous cell carcinoma cells (P0.05 or P0.01) and invasion ability (P0.05) were also significantly inhibited. The expression of E2F6 protein in cells transfected with pc DNA.3.1-E2F6 to miR-185 increased significantly (P0.01), and the cell proliferation P0.01) and invasiveness (P0.05) increased. Conclusion miR-185 can inhibit the proliferation and invasion of lung squamous cell carcinoma cells by targeting E 2 F 6.
【作者单位】：陕西省人民医院胸外科;陕西省新安中心医院妇产科;陕西省人民医院呼吸内科;
【基金】：陕西省自然科学基金面上项目(2014JM2-8166)~~
【分类号】：R734.2

【相似文献】