pH响应型近红外菁类荧光探针的合成及细胞成像

发布时间：2018-06-10 14:51

本文选题：荧光探针 + 不对称菁　；参考：《分析科学学报》2017年05期

【摘要】：癌症的早期诊治是提高癌症患者治愈率的关键。但传统的"always on"型显影剂存在自身背景干扰且易造成"假阳性"等问题。本文利用肿瘤细胞具有弱酸性这一特性,设计合成了p H激活型不对称菁类荧光探针,并选用氨基葡萄糖作为修饰基团以增强探针母体的水溶性并赋予其肿瘤靶向性。该探针具有p H调控的"off-on"可逆的近红外荧光特性,以及与肿瘤弱酸性微环境相吻合的p H响应范围。此外,探针的浓度高达1.0×10~(-5)mol/L时仍未表现出明显的细胞毒性。该探针在细胞水平实现了肿瘤细胞弱酸性微环境的特异性成像。
[Abstract]:Early diagnosis and treatment of cancer is the key to improve the cure rate of cancer patients. But the traditional "always on" type contrast agent has its own background interference and is easy to cause "false positive" and so on. In this paper, we designed and synthesized p-H-activated asymmetric cyanine fluorescent probes based on the weak acidity of tumor cells, and used glucosamine as the modified group to enhance the water solubility of the probe mother and endow it with tumor targeting. The probe has the reversible near infrared fluorescence characteristics of "off-on" regulated by pH and the pH response range consistent with the weak acidic microenvironment of the tumor. In addition, when the probe concentration was up to 1.0 脳 10 ~ (-5) mol / L, the cytotoxicity of the probe was not obvious. The probe achieves specific imaging of tumor cells in a weak acidic microenvironment at the cell level.
【作者单位】：食品科学与技术国家重点实验室(江南大学)江南大学食品学院分析食品安全学研究所;天津大学化工学院;天津化学化工协同创新中心;
【基金】：国家自然科学基金(No.21435001,21576194) 博士后科学基金(No.2015M581288)
【分类号】：O657.3;R730.4

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