雷替曲塞及其联合PD98059对HT-29细胞的影响

发布时间：2018-06-10 19:57

本文选题：结肠癌 + 雷替曲塞　；参考：《安徽医科大学学报》2017年05期

【摘要】：目的研究雷替曲塞及其联合PD98059对结肠癌细胞HT-29增殖及凋亡的影响,并探讨其分子机制。方法①在倒置显微镜下观察处理24 h后各组细胞的形态及数量变化;②使用细胞计数法检测不同处理条件下的细胞数;③使用流式细胞术检测各处理组细胞的凋亡率;④使用RT-PCR法检测各组K-RAS、ERK1、ERK2 mRNA的相对表达量。结果①倒置显微镜下观察可见与对照组相比,除PD98059组外,其他各组细胞数量均减少,形态均发生凋亡变化,且随雷替曲塞浓度增高,变化程度增加;联合组细胞形态及数量变化较对应雷替曲塞组程度更深。②细胞计数法显示各组细胞数均明显低于对照组的细胞数,且随着雷替曲塞浓度的增加,细胞数呈递减趋势;联合组与其他各组间均存在明显差异;③流式细胞仪检测显示和对照组相比,各组凋亡率均明显上升,在雷替曲塞组中存在浓度依赖性;联合组与其他各组的细胞凋亡率均存在差异;④ RT-PCR结果显示:与对照组相比,PD98059组、雷替曲塞组及联合组在K-RAS、ERK1、ERK2 mRNA的表达上均未表现出明显的差异。结论①雷替曲塞对结肠癌细胞HT-29增殖及凋亡的影响呈浓度依赖性;②雷替曲塞联合ERK抑制剂对HT-29细胞具有协同作用;③ ERK抑制剂不是在基因表达水平影响细胞的增殖及凋亡。
[Abstract]:Aim to study the effects of retitrexide and PD98059 on the proliferation and apoptosis of colon cancer cell line HT-29 and its molecular mechanism. Methods 1 the morphologic and quantitative changes of cells in each group were observed under inverted microscope for 24 h. Cell count was used to detect the number of cells under different treatment conditions and flow cytometry was used to detect the apoptosis rate of each treatment group. (4) the relative expression of ERK1 ERK2 mRNA in each group was detected by reverse transcription-polymerase chain reaction (RT-PCR). Results 1Compared with the control group under inverted microscope, the number of cells decreased and the morphology of all the cells in each group decreased, and the change degree increased with the increase of letitrexed concentration. The changes of cell morphology and quantity in the combined group were deeper than those in the corresponding group. 2 cell count method showed that the number of cells in each group was significantly lower than that in the control group, and the number of cells decreased with the increase of the concentration of letitrezel. Compared with the control group, the apoptosis rate of the combined group was significantly higher than that of the control group, and there was a concentration-dependent relationship between the combined group and the other groups. The results of RT-PCR showed that there was no significant difference in the expression of ERK1ERK2 mRNA between PD98059 group, Ratitrexed group and combined group. Conclusion (1) the effect of retitrexide on the proliferation and apoptosis of colon cancer cell line HT-29 was concentration-dependent. The synergistic effect of retitrexide combined with ERK inhibitor on HT-29 cell line was not at the level of gene expression, but also affected the proliferation and apoptosis of HT-29 cell line.
【作者单位】：安徽医科大学附属巢湖医院消化内科;
【基金】：安徽省高等学校自然科学研究项目(编号:KJ2015A334)
【分类号】：R735.35

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