姜黄素通过下调nectin-4抑制肺癌PC-9细胞的迁移和侵袭

发布时间：2018-06-12 01:24

本文选题：姜黄素 + 肺癌　；参考：《中国病理生理杂志》2017年02期

【摘要】：目的:探讨姜黄素对肺癌PC-9细胞迁移和侵袭的抑制作用及其与nectin-4表达的关系。方法:用MTT、划痕修复和Transwell小室实验检测姜黄素对肺癌PC-9细胞活力、迁移和侵袭能力的影响;用Western blot技术检测姜黄素对nectin-4表达及AKT通路的影响;经siRNA干扰nectin-4后观察姜黄素对PC-9细胞活力、迁移、侵袭及AKT通路的影响。结果:姜黄素能抑制PC-9细胞活力,10、20μmol/L姜黄素组与对照组相比,划痕修复率降低,穿膜细胞数目显著下降。姜黄素作用肺癌PC-9细胞24 h后,nectin-4表达下调。转染siNectin-4 48 h和72 h后,siNectin-4组比对照组的细胞活力显著降低(P0.01),划痕修复率及穿膜细胞数目显著下降(P0.01)。姜黄素与siNectin-4均抑制了PC-9细胞AKT通路的激活。姜黄素与siNectin-4联用时细胞活力降低(P0.01),划痕修复率、细胞侵袭能力及AKT磷酸化水平下降。结论:在肺癌PC-9细胞中,姜黄素通过下调nectin-4表达、调控下游AKT通路来抑制细胞迁移和侵袭。
[Abstract]:Aim: to investigate the inhibitory effect of curcumin on migration and invasion of lung cancer cell line PC-9 and its relationship with nectin-4 expression. Methods: the effects of curcumin on the viability, migration and invasion of lung cancer PC-9 cells were detected by MTT, scratch repair and Transwell chamber assay, and the effects of curcumin on nectin-4 expression and AKT pathway were detected by Western blot technique. The effects of curcumin on the viability, migration, invasion and AKT pathway of PC-9 cells were observed by siRNA interference with nectin-4. Results: compared with the control group, curcumin could inhibit the viability of PC-9 cells by 10 ~ 20 渭 mol / L curcumin. The rate of scratch repair and the number of perforating cells were significantly decreased. The expression of nectin-4 was down-regulated by curcumin on PC-9 cells for 24 h. After transfection of siNectin-4 for 48 h and 72 h, the cell viability of siNectin-4 group was significantly lower than that of control group, while the rate of scratch repair and the number of perforating cells were significantly decreased. Curcumin and siNectin-4 inhibited the activation of AKT pathway in PC-9 cells. Curcumin combined with siNectin-4 decreased cell viability, scratch repair rate, cell invasion ability and AKT phosphorylation level. Conclusion: curcumin inhibits cell migration and invasion by down-regulation of nectin-4 expression and regulation of downstream AKT pathway in PC-9 cells.
【作者单位】：中国人民解放军第一一七医院呼吸内科;
【基金】：浙江省医药卫生科技计划(No.2011KYA139) 浙江省科技厅公益性技术应用研究计划(No.2014C33277) 杭州市科技发展计划(No.20130633B29;No.20140633B40) 浙江省自然科学基金资助项目(No.LY12H16001)
【分类号】：R734.2

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