黄芪多糖联合顺铂抑制小鼠Lewis肺癌移植瘤生长及其机制

发布时间：2018-06-14 08:00

本文选题：黄芪多糖(APS) + Lewis肺癌　；参考：《细胞与分子免疫学杂志》2017年04期

【摘要】：目的观察黄芪多糖(APS)及联合顺铂(DDP)对Lewis肺癌(LLC)移植瘤小鼠凋亡蛋白细胞色素C(CytC)和高温必需丝氨酸蛋白酶A2(Omi/HtrA2)表达的影响。方法 90只C57BL/6J小鼠,随机分为正常组、模型组、(50、100、200)μg/mL APS处理组、6 mg/kg DDP处理组、3 mg/kg DDP联合(50、100、200)μg/mL APS处理组,每组10只。除正常组外,其余80只均接种1×107LLC细胞0.2 mL于右前肢腋窝皮下,建立荷瘤小鼠模型。造模次日起,治疗组的小鼠给予腹腔注射0.3 mL药物。每周注射1次DDP,其余药物每天1次,正常组和模型组注射等体积生理盐水,连续20 d,于第21天处死。采用HE染色观察肿瘤组织病变情况;免疫组织化学染色和图像分析法检测移植瘤细胞中的Cyt C及Omi/HtrA2的表达和定位。结果 (100、200)μg/mL APS处理及3 mg/kg DDP联合(100、200)μg/mL APS的荷瘤小鼠肿瘤质量减轻。与模型组小鼠比较,200μg/mL APS联合3 mg/kg DDP小鼠的肿瘤细胞坏死最为明显,各处理组的肿瘤组织中Cyt C、Omi/Htr A2蛋白水平均增加,200μg/mL APS联合3 mg/kg DDP小鼠的肿瘤组织增加最明显。结论APS及联合DDP能抑制小鼠Lewis肺癌细胞的生长,可能与肿瘤细胞Cyt C、Omi/Htr A2表达增加有关。
[Abstract]:Objective to observe the effects of Astragalus Polysaccharide (APS) combined with cisplatin (DDP) on the expression of cytochrome C (Cyt C) and high temperature essential serine protease A2OmiP HtrA2 in mice transplanted with Lewis lung cancer (LLC). Methods 90 C57BL / 6J mice were randomly divided into normal group (n = 10), model group (n = 10) treated with 5 渭 g / mL APS (n = 10), group treated with 3 mg/kg / mL DDP (n = 10) and treated with 5 渭 g / mL APS for 6 mg/kg / mL (n = 10). In addition to the normal group, the other 80 mice were inoculated with 0.2 mL of 1 脳 107 LLC cells in the subcutaneous armpit of the right forelimb to establish the tumor-bearing mice model. Mice in the treatment group were given 0.3 mL of drug intraperitoneally since the next day. DDP was injected once a week, the other drugs were injected once a day, the normal group and the model group were injected with the same volume of normal saline for 20 days, and were killed on the 21st day. Immunohistochemical staining and image analysis were used to detect the expression and localization of Cyt C and Omip HtrA2 in transplanted tumor cells. Results the tumor quality of tumor-bearing mice treated with 100200 渭 g / mL APS and 3 mg/kg DDP combined with 100200 渭 g / mL APS decreased. Compared with the model group, the tumor cell necrosis was the most obvious in 渭 g / mL APS combined with 3 mg/kg DDP mice, and the tumor tissue was increased most obviously in the tumor tissue of each treatment group. The protein level of Cyt Con Omir-Htr _ 2 was increased by 200 渭 g / mL APS combined with 3 mg/kg DDP. Conclusion APS and DDP can inhibit the growth of mouse Lewis lung cancer cells, which may be related to the increased expression of Cyt Con OmiP / Htr A2.
【作者单位】：甘肃中医药大学;甘肃省高校重大疾病分子医学与中医药防治研究重点实验室;甘肃省中药药理与毒理学重点实验室;
【基金】：甘肃省高校基本科研业务项目(2014) 甘肃省科技厅计划项目(148RJZA060) 兰州市科技局计划项目(2014-1-23)
【分类号】：R734.2

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