CyclinD1和TK1在乳腺癌的表达及意义

发布时间：2018-06-15 08:42

本文选题：Cyclin + D1　；参考：《青岛大学》2017年硕士论文

【摘要】：目的:(1)探讨乳腺癌低危组、中危组、高危组患者中细胞周期蛋白(Cyclin D1)以及胸腺激酶1(TK1)的不同表达情况;(2)比较Cyclin D1以及TK1与患者年龄的大小、淋巴结是否转移、肿瘤的大小、TNM分期、组织学的分级表达、ER、PR、C-erb B-2等临床病理参数的关系;(3)探讨Cyclin D1以及TK1的表达与乳腺癌的临床意义。方法:(1)选择乳腺手术切除的病例标本共计87例,均来自文登区人民医院普外科,并且已取得患者及家属的同意,签定文登区人民医院的相关知情文件的同意书,且通过文登区人民医院的伦理委员会的标准。(2)常规病理检查结果采用半定量的组织学分级的方法判断,然后按2005St.Gallen国际乳腺癌治疗共识按患者年龄、临床分期、腋窝淋巴结转移、病理学的分级HER-2表达等指标将患有乳腺恶性肿瘤的病人分为低危组、中危组、高危组三组。(3)各病理类型组织均是采用免疫组化的方法检测Cyclin D1以及TK1的表达情况;比较Cyclin D1以及TK1在乳腺恶性肿瘤低危组、中危组、高危组三组的表达情况;比较Cyclin D1以及TK1二者与临床各病理学指标(比如:患者的年龄大小、淋巴结是否转移、肿瘤的大小、TNM分期、组织学的分级表达、ER、PR、C-erb B-2)的关系。(4)运用SPSS17.0统计软件包对Cyclin D1以及TK1与乳腺癌病人临床病理参数之间的关系以及两种对乳腺癌预后的影响进行分析。结果:(1)Cyclin D1阳性(+)为细胞核染色,阳性的表达率为83.9%(73/87)。Cyclin D1阳性(+)表达的结果与ER有明显差异(P0.05);但与患者年龄的大小、淋巴结是否转移、肿瘤的大小、TNM分期、组织学的分级表达、PR、C-erb B-2没有相关性(P0.05)。在乳腺癌患者低危组、中危组、高危组中Cyclin D1表达水平由低到高依次为低危组中危组高危组。(2)TK1阳性(+)为细胞质染色,阳性的表达率为77%(67/87)。TK1阳性(+)表达与与组织学分级表达有差异(P0.05);但与患者的年龄大小、淋巴结是否转移、肿瘤的大小、TNM分期、ER、PR、C-erb B-2无相关性(P0.05)。在乳腺癌患者低危组、中危组、高危组中TK1表达水平由低到高依次为低危组中危组高危组。结论:Cyclin D1和TK1在乳腺癌组织中呈高表达状态,Cyclin D1和TK1呈现明显正相关关系,提示Cyclin D1和TK1在乳腺癌的发生、发展过程中有意义。
[Abstract]:Objective to investigate the different expression of cyclin D1 and TK1 in low risk group, moderate risk group, high risk group and high risk group. The clinical significance of the expression of Cyclin D1 and TK1 in breast cancer was studied. Methods A total of 87 cases of breast surgery were selected, all from the Department of General surgery of the people's Hospital of Wenten District. The consent of the patients and their families was obtained, and the consent of the relevant informed documents of the people's Hospital of Wenteng District was signed. The results of routine pathological examination were judged by semi-quantitative histological grading, and then according to the international consensus of 2005St.Gallen, according to the age, clinical stage, axillary lymph node metastasis, the results of routine pathological examination were judged by the standard of ethics committee of Wendeng District people's Hospital. The patients with breast cancer were divided into three groups: low risk group, middle risk group and high risk group. The expression of Cyclin D1 and TK1 was detected by immunohistochemical method. To compare the expression of Cyclin D1 and TK1 in low risk group, middle risk group and high risk group, and to compare the expression of Cyclin D1 and TK1 with the clinicopathological indexes (such as age, lymph node metastasis or not). The relationship between tumor size and TNM stage, histological grade expression of ERA PRERB B 2) and the relationship between Cyclin D1 and TK1 and the clinicopathological parameters of breast cancer patients and their influence on the prognosis of breast cancer were analyzed by SPSS 17.0 software package. Results the positive expression rate of Cyclin D1 was 83.9% and the positive rate of Cyclin D1 was significantly different from that of ER (P 0.05), but it was significantly different from that of ER in age, lymph node metastasis, tumor size and TNM stage. The histologically graded expression of PRA C-erb B-2 was not correlated with P0.05. In low risk group, moderate risk group and high risk group, the expression of Cyclin D1 in low risk group, middle risk group and high risk group was in order of Cyclin D1 positive staining in low risk group, middle risk group, high risk group and high risk group. The positive expression rate of TK1 was 77 / 87. TK1 positive expression was significantly different from histological grade expression (P 0.05), but had no correlation with age, lymph node metastasis, tumor size, TNM stage, ERP PRA C-erb B-2, and P0.05P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05 and P 0. 05, respectively. The expression of TK1 in low risk group, middle risk group and high risk group was in order of low risk group, middle risk group and high risk group. Conclusion there is a significant positive correlation between Cyclin D1 and TK1 in breast cancer tissues, suggesting that Cyclin D1 and TK1 may play an important role in the occurrence and development of breast cancer.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.9

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