Sox4在女性肺癌细胞上皮-间质转化中的作用及其机制

发布时间：2018-06-15 19:26

本文选题：Sox + 肺癌　；参考：《中国老年学杂志》2017年04期

【摘要】：目的探讨Sox4在女性肺癌细胞上皮-间质转化(EMT)中的作用及其机制。方法 76例行肺癌根治术的女性肺癌组织标本为观察组,49例癌旁肺组织标本为对照组,采用实时荧光定量聚合酶链反应(q PCR)和Western印迹法检测Sox4的表达水平;采用RNA干扰技术下调Sox4表达,利用脂质体2000向肺癌细胞系XWLC-05转染Sox4-si RNA,同时以未转染XWLC-05为对照,采用q PCR和Western印迹方法检测两组Sox4表达水平及内镜下黏膜切除术(EMR)上皮标记β-catenin、E-cadherin,间质标记Fibronectin、vimentin、N-cadherin的表达情况,采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氯唑溴盐(MTT)、Transwell及细胞划痕实验检测转染组与未转染组细胞增殖、侵袭、迁移能力。结果观察组Sox4 m RNA水平及蛋白水平表达均明显高于对照组(P0.05);转染组Sox4 m RNA水平及蛋白水平表达均明显低于未转染组(P0.05);q PCR结果显示,转染组β-catenin、E-cadherin水平明显高于未转染组(P0.05),转染组Fibronectin、vimentin、N-cadherin水平明显低于未转染组(P0.05),Western印迹结果与q PCR检测结果相一致;MTT实验结果显示,转染Sox4-si RNA细胞后,XWLC-05细胞增殖能力与未转染组无明显差异(P0.05),Transwell实验结果显示,转染Sox4-si RNA细胞后,XWLC-05细胞侵袭、迁移能力较未转染组明显降低。结论 Sox4可能是通过下调β-catenin、E-cadherin表达及上调Fibronectin、vimentin、N-cadherin表达促进EMT发生,诱导人肺癌细胞发生侵袭转移。
[Abstract]:Objective to investigate the role and mechanism of Sox4 in epithelial-mesenchymal transformation (EMTT) of female lung cancer cells. Methods the expression of Sox4 was detected by real-time quantitative polymerase chain reaction (PCR) and Western blotting (Western blot) in 76 female lung cancer tissues which were treated with radical resection of lung cancer as control group (n = 49) and control group (n = 49), and the expression of Sox4 was detected by real-time fluorescence quantitative polymerase chain reaction (PCR) and Western blotting. The expression of Sox4 was down-regulated by RNA interference technique, and Sox4-si RNAs were transfected into lung cancer cell line XWLC-05 by liposome 2000, while non-transfected XWLC-05 was used as control. The expression of Sox4, 尾 -cateninine E-cadherin and fibronectin vimentinine N-cadherin were detected by Q PCR and Western blot. The cell proliferation, invasion and migration ability of the transfected and untransfected cells were detected by the method of 3-titrazolium 5-dimethylthiazole-2-diphenyltetrazolium bromide (MTT) -5-dimethylthiazolium (5-dimethylthiazolium) -2-diphenyltetrazolium bromide (MTT) and the cell scratch test. Results the expression of Sox4 mRNA and protein in the observation group was significantly higher than that in the control group, and the expression of Sox4 mRNA and protein in the transfected group was significantly lower than that in the untransfected group. The level of 尾 -cateninine E-cadherin in the transfected group was significantly higher than that in the non-transfected group (P 0.05), and the level of fibronectinine vimentin N-cadherin in the transfected group was significantly lower than that in the untransfected group. The results of Western blot and Q PCR showed that the results of MTT assay showed that the level of 尾 -cateninine E-cadherin in the transfected group was significantly higher than that in the non-transfected group. After transfection of Sox4-si RNA cells, the proliferative ability of XWLC-05 cells was not significantly different from that of non-transfected cells. The results showed that after transfection of Sox4-si RNA cells, XWLC-05 cells invaded, and the migration ability of XWLC-05 cells was significantly lower than that of untransfected cells. Conclusion Sox4 may promote EMT by down-regulating the expression of 尾 -cateninine E-cadherin and up-regulating the expression of fibronectinin N-cadherin, and induce invasion and metastasis of human lung cancer cells.
【作者单位】：昆明医科大学第三附属医院;
【基金】：国家自然科学基金地区基金(No.81460441) 云南省教育厅科研基金重点项目(No.2013Z110) 云南省科技厅-昆明医科大学联合专项基金项目(No.2013FB132)
【分类号】：R735.2

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