IL-33在胰腺癌中的表达及临床意义

发布时间：2018-06-15 20:36

本文选题：IL-33 + 胰腺癌　；参考：《苏州大学》2016年硕士论文

【摘要】：目的:分析胰腺癌患者肿瘤组织、癌旁组织以及正常胰腺组织中IL-33的表达差异;初步探讨IL-33的表达与胰腺癌临床病理特征的相关性。方法:利用qRT-PCR法检测经临床病理学确诊的胰腺癌患者的肿瘤组织、癌旁组织以及正常胰腺组织中的IL-33 mRNA的相对表达量。同时运用免疫组织化学方法分析以上相应胰腺癌患者的肿瘤组织、癌旁组织、正常胰腺组织IL-33的表达情况。探讨在癌旁组织中IL-33 mRNA表达量与患者患者的年龄、性别、肿瘤大小、分化程度、TNM分期、淋巴结转移等临床病理特征之间的关系。结果:qPCR结果经统计学软件处理分析显示:IL-33 mRNA在胰腺癌癌旁组织的表达量最高,正常胰腺组织次之,癌组织中表达最少;免疫组化结果提示IL-33在癌旁组织中表达最高,正常组织中表达次之,肿瘤组织表达最少;在癌旁组织中,IL-33mRNA的表达与淋巴结转移和局部邻近器官侵犯呈明显负相关(P?0.05),与胰腺癌患者的性别、年龄、肿瘤的大小、分化程度、TNM分期无明显相关性(P0.05)。结论:IL-33在胰腺癌癌旁组织表达最高,正常组织中表达次之,肿瘤组织中最少。在癌旁组织中,IL-33的表达与胰腺癌的淋巴结转移和局部邻近器官侵犯呈负相关,提示IL-33在胰腺癌进展中具有抵抗肿瘤侵袭转移的作用。IL-33表达与患者年龄、性别、肿瘤大小、分化程度、TNM分期无关。
[Abstract]:Objective: to analyze the difference of IL-33 expression in tumor tissues, paracancerous tissues and normal pancreatic tissues in patients with pancreatic cancer, and to explore the relationship between the expression of IL-33 and clinicopathological features of pancreatic cancer. Methods: the relative expression of IL-33 mRNA in tumor tissues, adjacent tissues and normal pancreas tissues was detected by qRT-PCR. The expression of IL-33 in tumor tissues, paracancerous tissues and normal pancreas tissues was analyzed by immunohistochemical method. To investigate the relationship between the expression of IL-33 mRNA in paracancerous tissues and clinicopathological features such as age, sex, tumor size, degree of differentiation, TNM stage, lymph node metastasis and so on. Results by statistical software analysis, the expression of Wil 33 mRNA was the highest in the adjacent tissues of pancreatic cancer, followed by the normal pancreatic tissues, and the lowest in the cancer tissues, and the expression of IL-33 was the highest in the paracancerous tissues of pancreatic cancer by immunohistochemistry, and the results of immunohistochemistry showed that the expression of IL-33 mRNA was the highest in the paracancerous tissues. The expression of IL-33 mRNA in adjacent tissues was negatively correlated with lymph node metastasis and local adjacent organ invasion, and was negatively correlated with sex, age and tumor size of pancreatic cancer patients, while the expression of IL-33 mRNA in normal tissues was the second, and the expression of IL-33 mRNA in tumor tissues was the least, and the expression of IL-33 mRNA was negatively correlated with lymph node metastasis and invasion of local adjacent organs. There was no significant correlation between TNM stage and differentiation degree (P 0.05). Conclusion the expression of 10-IL-33 is the highest in the adjacent tissues of pancreatic cancer, followed by the normal tissues, and the least in the tumor tissues. The expression of IL-33 in paracancerous tissues was negatively correlated with lymph node metastasis and local adjacent organ invasion of pancreatic carcinoma. It suggested that IL-33 may resist invasion and metastasis of pancreatic cancer, and the expression of IL-33 was associated with age, sex and tumor size. The degree of differentiation was not related to TNM stage.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R735.9

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