BDNF、Ets-1在卵巢癌的表达情况及临床意义

发布时间：2018-06-17 10:05

本文选题：BDNF + Ets-1　；参考：《青岛大学》2017年硕士论文

【摘要】：目的:该研究主要是进一步论证与卵巢癌侵袭转移相关的BDNF、Ets-1这两个基因蛋白在上皮性浆液性卵巢癌的表达情况与临床病理特征之间的关系及两者之间的相关性。研究BDNF、Ets-1在上皮性浆液性卵巢癌的发生及发展关系。方法:通过免疫组化及Western Blot方法检测BDNF、Ets-1这两个基因蛋白在上皮性浆液性卵巢癌石蜡切片及新鲜卵巢组织中的表达情况,分析BDNF、Ets-1与临床病理特征(临床分期、分化程度、淋巴结转移、远处转移、年龄等)的关系,及进一步研究两种基因蛋白在上皮性浆液性卵巢癌中表达的相关性。结果:1.BDNF在正常卵巢组织中的的阳性表达率为15%,在交界性卵巢组织中阳性表达率为40%,在上皮性浆液性卵巢癌的阳性表达率为67%,主要位于细胞质中,由此可见在恶性卵巢组织中的阳性表达率最高,差异有统计学意义(P0.05)。2.BDNF在浆液性卵巢癌中的表达与临床分期(P=0.001)、有无远处转移(P=0.004)、有无淋巴结转移(P=0.000)及组织分化程度(P=0.044)有关,差异有统计学意义,(P0.05),与年龄无明显关系(P0.05)。BDNF在临床分期晚期、有远处转移、有淋巴结转移、组织分化程度低组中呈高表达。BDNF在临床分期早期、无远处转移、无淋巴结转移、组织分化程度高组中表达水平低。3.Ets-1在卵巢良性组织中阳性表达率为22%,在交界性卵巢组织中阳性表达率为44%,在上皮性浆液性卵巢癌组织中阳性表达率为50%,主要位于细胞间质中,少量位于细胞核中。可以看出,Ets-1在三种不同卵巢组织中表达情况存在明显差距,三组之间X2=2.0,P=0.000,差异有统计学意义(P0.05)。4.Ets-1在浆液性卵巢癌中的表达与临床分期(P=0.024)、有无远处转移(P=0.000)、有无淋巴结转移(P=0.003)及组织分化程度(P=0.001)有关,差异有统计学意义,(P0.005),与年龄无明显关系(P0.05)。Ets-1在临床分期晚期、有远处转移、有淋巴结转移、组织分化程度低组中呈高表达。Ets-1在临床分期早期、无远处转移、无淋巴结转移、组织分化程度高组中表达水平低或几乎无表达。5.经过Spearman相关性分析,在上皮性浆液性卵巢癌组织中BDNF表达强度增加,Ets-1表达强度也增加,且随着卵巢癌恶性程度的增加,BDNF、Ets-1两个基因蛋白表达也增加,P=0.041,两者在上皮性浆液性卵巢癌组织的表达成正相关(r=0.447,P0.05)。结论:1.BDNF、Ets-1这两个基因蛋白在上皮性浆液性卵巢癌组织中高表达,且随着恶性程度的增加,表达水平也升高,说明BDNF、Ets-1可能参与了卵巢癌的发生及发展过程。2.BDNF、Ets-1表达水平与卵巢癌患者的预后因素有关,说明BDNF、Ets-1可能影响患者的预后。抑制这两个基因蛋白的表达将来可能成为治疗卵巢癌的一个重要的治疗方法。3.经过Spearman相关性分析,在上皮性浆液性卵巢癌组织中BDNF表达强度增加,Ets-1表达强度也增加,且随着卵巢癌恶性程度的增加,BDNF、Ets-1两个基因蛋白表达也增加,两者在上皮性浆液性卵巢癌组织的表达成正相关。
[Abstract]:Objective: This study is to further demonstrate the relationship between the expression of BDNF, Ets-1 and the clinicopathological features of the two gene proteins associated with the invasion and metastasis of ovarian cancer, and the relationship between them and the relationship between them. The relationship between the development and development of BDNF and Ets-1 in epithelial serous ovarian cancer is studied. Methods: Immunohistochemical and Western Blot methods were used to detect the expression of BDNF, Ets-1, two gene proteins in the paraffin and fresh ovarian tissues of epithelial serous ovarian cancer, and to analyze the relationship between BDNF, Ets-1 and clinicopathological features (clinical stage, differentiation, lymph node metastasis, distant migration, age, etc.), and further study the two kinds of gene eggs. The positive expression rate of 1.BDNF in normal ovarian tissue was 15%, the positive expression rate in the borderline ovarian tissue was 40%, the positive expression rate in epithelial serous ovarian cancer was 67%, mainly in the cytoplasm, thus the positive form in the malignant ovarian tissue was found. The difference was statistically significant (P0.05), the expression of.2.BDNF in serous ovarian cancer and clinical stage (P=0.001), distant metastasis (P=0.004), lymph node metastasis (P=0.000) and the degree of tissue differentiation (P=0.044), the difference was statistically significant, (P0.05), and there was no significant relationship with age (P0.05).BDNF in the late stage of clinical stage. The positive expression rate of.BDNF in the benign tissue of the ovary was 22%, the positive rate in the borderline ovarian tissue was 44%, and the positive expression rate in the borderline ovarian tissue was 44%, in the epithelial serous sex. The positive expression rate in ovarian cancer tissues was 50%, mainly in the cytoplasm and a small amount in the nucleus. It can be seen that the expression of Ets-1 in three different ovarian tissues has a significant difference, and the difference between the three groups is X2=2.0 and P=0.000, the difference is statistically significant (P0.05), the expression of.4.Ets-1 in the serous ovarian cancer and the clinical stage (P=0.024). No distant metastasis (P=0.000) was associated with lymph node metastasis (P=0.003) and the degree of tissue differentiation (P=0.001). The difference was statistically significant, (P0.005), and there was no significant relationship with age (P0.05).Ets-1 in the late stage of clinical stage, with distant metastasis, lymph node metastasis, and high expression of.Ets-1 in the low level of tissue differentiation in the early stage of clinical staging and no distant transition. The expression of BDNF in the epithelial serous ovarian cancer tissues increased and the expression of Ets-1 increased, and the expression of Ets-1 was increased with the increase of the malignant degree of ovarian cancer, and the expression of two gene proteins in BDNF and Ets-1 increased with the increase of the malignant degree of ovarian cancer, and the expression of two gene proteins in BDNF and Ets-1 also increased, P=0.041, The expression of the two in epithelial serous ovarian cancer tissue is positive correlation (r=0.447, P0.05). Conclusion: 1.BDNF, Ets-1 these two gene proteins are highly expressed in epithelial serous ovarian cancer tissue, and with the increase of the malignant degree, the expression level is also elevated, indicating that BDNF, Ets-1 may be involved in the development and development of ovarian cancer.2.BDNF, Ets-1 Expression level is associated with prognostic factors in ovarian cancer patients, indicating that BDNF, Ets-1 may affect the prognosis of patients. Inhibition of the expression of these two gene proteins may become an important treatment for ovarian cancer in the future,.3. after Spearman correlation analysis, the increase of BDNF expression in epithelial serous ovarian cancer, Ets-1 table With the increase of the malignant degree of ovarian cancer, the expression of the two gene proteins of BDNF and Ets-1 also increased, which was positively related to the expression of epithelial serous ovarian cancer.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.31

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