卵巢癌生物标志物的糖链谱研究进展

发布时间：2018-06-19 16:49

本文选题：卵巢癌 + 糖组学　；参考：《生物化学与生物物理进展》2017年10期

【摘要】：糖类抗原125(CA125)被认为是卵巢癌诊断的"金标准",但在临床应用中普遍存在着特异性不高的问题.肿瘤形成和发展过程中常伴有糖基化修饰异常和糖链结构的改变,不同的肿瘤具有特异的异常糖链结构.近年来,借助凝集素芯片、多重质谱分析等糖蛋白组学和糖组学研究技术,发现不同来源CA125的O-糖链和N-糖链结构存在着明显的微观不均一性,以这些特征性糖链结构为标志物,可以显著提高CA125对卵巢癌的诊断特异性.在过去的10年,研究者们除对CA125糖链结构和糖基化模式做了深入的研究外,还利用糖组的研究方法,直接对来自卵巢癌患者血液、体液(腹水、囊泡液等)中糖蛋白的糖链做了精细的结构解析,结果显示,可有效鉴别卵巢癌患者和健康志愿者的特异性N-糖链结构,有可能成为灵敏度高和特异性好的卵巢癌生物标志物.卵巢癌生物标志物研究发展的总趋势是从传统的对蛋白质的定性和定量研究,逐步转向于对标志物糖基化修饰和特异性糖链结构的鉴定以及定量分析.本文从糖组学的视角,对卵巢癌标志物糖组学的研究现状及发展趋势进行了综述和展望.
[Abstract]:Carbohydrate antigen 125 (CA125) is considered to be the gold standard for the diagnosis of ovarian cancer, but the specificity is not high in clinical application. The abnormal glycosylation modification and the change of glycosylated structure are often associated with the formation and development of tumor, and different tumors have special abnormal glycosylated structure. In recent years, by means of lectin chip, multiplex mass spectrometry and other techniques of glycoproteomics and glycometry, it was found that the structure of O-sugar chain and N-sugar chain of CA125 from different sources had obvious microscopic heterogeneity. Using these characteristic sugar chain structures as markers, CA125 can significantly improve the diagnostic specificity of ovarian cancer. Over the past 10 years, researchers have done in-depth research on the structure and glycosylation patterns of the CA125 sugar chain, using the sugar group method to directly study blood and body fluids (ascites) from patients with ovarian cancer. The results show that it can effectively identify the specific N-sugar chain structure of ovarian cancer patients and healthy volunteers, and may become a highly sensitive and specific biomarker of ovarian cancer. The general trend of ovarian cancer biomarkers is from the traditional qualitative and quantitative studies on proteins to the identification and quantitative analysis of glycosylation modification and specific glycation structure of ovarian cancer biomarkers. From the perspective of glycometry, the present situation and development trend of glycomics of ovarian cancer markers were reviewed and prospected.
【作者单位】：西北大学生命科学学院功能糖组学实验室;第四军医大学唐都医院妇产科;
【基金】：国家自然科学基金资助项目(81372365)~~
【分类号】：R737.31

【相似文献】