MicroRNA-155在肝细胞癌对索拉非尼抗药中的作用研究

发布时间：2018-06-22 19:59

本文选题：微小RNA + 肝细胞癌　；参考：《中国药理学通报》2017年05期

【摘要】：目的探讨微小RNA155(microRNA-155,miR-155)在肝细胞癌(hepatocellular carcinoma,HCC)对索拉非尼(sorafenib)抗药中的作用。方法将miR-155抑制慢病毒(miR-155 inhibitor)转染miR-155表达相对较高的SMMC-7721细胞,而miR-155过表达慢病毒(miR-155)转染miR-155表达相对较低的Hep G2细胞;用荧光定量PCR(qPCR)检测经慢病毒转染的SMMC-7721细胞及HepG2细胞miR-155表达量,以验证慢病毒转染效果;通过CCK-8法及流式细胞术检测各组细胞经索拉非尼作用后的存活率及凋亡情况;用Western blot检测凋亡相关蛋白活性caspase-3表达量,从而进一步探究各组细胞凋亡情况。结果与对照组相比,转染miR-155抑制慢病毒的SMMC-7721细胞表达miR-155明显下调(P0.01),经索拉非尼处理后其存活率明显降低(P0.05),而索拉非尼诱导其凋亡明显增加(P0.01),其活性caspase-3表达量明显上调(P0.01);miR-155过表达慢病毒转染HepG2细胞后,则相反。结论 miR-155参与肝细胞癌对索拉非尼的抗药,有希望成为一个肝癌治疗的新靶标。
[Abstract]:Objective to investigate the role of microRNA-155 miR-155 in the resistance of hepatocellular carcinoma to Solafenil (sorafenib). Methods miR-155 inhibited lentivirus (miR-155 inhibitor) was transfected into SMMC-7721 cells with relatively high miR-155 expression, while miR-155 overexpression lentivirus (miR-155) was transfected into Hep G2 cells with relatively low miR-155 expression. The expression of miR-155 in SMMC-7721 cells and HepG2 cells was detected by fluorescence quantitative polymerase chain reaction (qPCR). In order to verify the effect of lentivirus transfection, CCK-8 and flow cytometry were used to detect the survival rate and apoptosis of the cells treated with Solafenil, and Western blot was used to detect the expression of apoptosis-related protein (caspase-3). So as to further explore the apoptosis of each group. Results compared with the control group, The expression of miR-155 was down-regulated (P0.01) in SMMC-7721 cells transfected with miR-155. The survival rate of SMMC-7721 cells treated with sorafenil was significantly decreased (P0.05), while the apoptosis induced by sorafenil was significantly increased (P0.01). The expression of active caspase-3 was up-regulated (P0.01) and the overexpression of miR-155 was up-regulated (P0.01) in HepG2 cells. The opposite is true. Conclusion miR-155 may be a new target for the treatment of hepatocellular carcinoma.
【作者单位】：重庆医科大学附属第一医院肝胆外科;
【基金】：国家自然科学基金资助项目(No 81470898)
【分类号】：R735.7

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