小干扰RNA沉默CXCR4和CXCR7对人子宫内膜癌Ishikawa细胞裸鼠移植瘤生长的抑制作用

发布时间：2018-06-22 23:08

本文选题：siRNA + CXCR　；参考：《中国肿瘤生物治疗杂志》2017年04期

【摘要】：目的:探讨针对趋化因子受体基因CXCR4和CXCR7的小干扰RNA(siRNA)对人子宫内膜癌Ishikawa细胞裸鼠移植瘤生长的影响。方法:将子宫内膜癌Ishikawa细胞株注射于裸鼠肩胛背部皮下建立动物模型,待肿瘤最长径达5~7mm后,将所有荷瘤裸鼠随机分组,分别给予CXCR4-siRNA和CXCR7-siRNA单独或联合瘤体内注射,并以阴性对照siRNA和生理盐水作为对照组,每3 d治疗一次,共6个治疗周期。观察各组裸鼠移植瘤的生长,比较各组移植瘤的体积和质量的差异,并以实时荧光定量RT-PCR、Western blotting和免疫组化技术验证CXCR4和CXCR7的基因沉默效果。结果:成功建立Ishikawa细胞裸鼠移植瘤模型,与阴性对照siRNA组和空白对照组相比,CXCR4 siRNA治疗组、CXCR-siRNA治疗组和CXCR4-siRNA+CXCR7-siRNA治疗组的肿瘤的生长均明显受到抑制(均P0.05);CXCR4-siRNA和/或CXCR7-siRNA瘤体内直接注射能显著下调CXCR4、CXCR7 mRNA(均P0.05)和蛋白(均P0.01)的表达。结论:siRNA干扰CXCR4和CXCR7表达能够有效抑制人子宫内膜癌Ishikawa细胞裸鼠移植瘤的生长。
[Abstract]:Aim: to investigate the effects of small interfering RNA (siRNA) targeting chemokine receptor genes CXCR4 and CXCR7 on the growth of human endometrial carcinoma cell line Ishikawa in nude mice. Methods: Ishikawa cell line of endometrial carcinoma was injected subcutaneously into the dorsal scapula of nude mice to establish an animal model. After the tumor reached the maximum diameter of 5~7mm, all the nude mice were randomly divided into two groups: CXCR4-siRNA and CXCR7-siRNA were injected separately or intraperitoneally. The control group was treated with siRNA and normal saline every 3 days for 6 cycles. The growth of xenografts in nude mice was observed, the volume and quality of transplanted tumors were compared, and the gene silencing effect of CXCR4 and CXCR7 was verified by real-time fluorescence quantitative RT-PCR Western blotting and immunohistochemical technique. Results: Ishikawa cell model was successfully established in nude mice. Compared with negative control siRNA group and blank control group, CXCR4 siRNA treatment group and CXCR4-siRNA CXCR7-siRNA group were significantly inhibited from tumor growth (P0.05) and / or CXCR7-siRNA injection could significantly down-regulate the expression of CXCR4CXCR7 mRNA (P0.05) and CXCR7-siRNA protein (P0.01). Conclusion interfering with the expression of CXCR4 and CXCR7 can effectively inhibit the growth of human endometrial carcinoma cell line Ishikawa in nude mice.
【作者单位】：青岛大学医学部;青岛市妇女儿童医院妇科;
【基金】：山东省优秀中青年科学家科研奖励基金资助项目(No.BS2009SW002) 山东省自然科学基金资助项目(No.ZR2013HM012)~~
【分类号】：R730.5;;R737.33

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