TTF-1和Ki-67在非小细胞肺癌中的表达及意义

发布时间：2018-06-23 21:55

本文选题：非小细胞肺癌 + 甲状腺转录因子-1　；参考：《皖南医学院》2017年硕士论文

【摘要】：目的:本研究通过检测非小细胞肺癌(non-small cell lung cancer,NSCLC)中甲状腺转录因子-1(Thyroid Transcription Factor-1,TTF-1)和Ki-67(细胞增殖核抗原)的表达,并统计它们各自与患者各临床指标的关系,然后通过统计软件检测两者之间的关系,以便为认识和了解NSCLC的特性提供一定的帮助。方法:选取皖南医学院弋矶山医院2015.1-2016.6住院在呼吸内科和胸心外科的非小细胞肺癌患者(组织标本来源途径分别为支气管镜活检标本,CT引导下穿刺活检标本和外科手术切除标本,确诊方法为病理学检查),例数为130例。通过免疫组化(Immunohistochemistry,IHC)的方法测定这些患者组织标本中的TTF-1和Ki-67表达情况,来探讨两者各自与患者临床特征的关系,并测定TTF-1和Ki-67两者的关系。统计软件用SPSS17.0。统计TTF-1和Ki-67的与各参数的关系时用卡方检验、统计TTF-1和Ki-67的相关性时用秩相关检验。结果:1.TTF-1在腺癌、女性、中高分化、I-II期的NSCLC患者中阳性率高,而与NSCLC患者的年龄、淋巴结转移情况、有无吸烟等无关。2.Ki-67在有吸烟史、低分化、有淋巴结转移的NSCLC患者中阳性率高,而与病理类型、性别、年龄、分期无关。3.在NSCLC中,TTF-1和Ki-67两者的表达无明显相关性(r=0.173,p0.05)。结论:1、TTT-1对于诊断肺腺癌有较积极的意义。2、在NSCLC患者当中女性、分化程度越高,临床分期越早,甲状腺转录因子-1表达阳性率越高。TTF-1可能参与NSCLC的侵袭,可能在NSCLC侵袭通路中的某一环节扮演着重要作用。3、在NSCLC患者当中吸烟患者、分化程度越低差、淋巴结转移患者,Ki-67表达阳性率越高。Ki-67可能参与NSCLC的浸润、转移,可能在NSCLC的浸润和转移通路中的某一环节扮演着重要作用。
[Abstract]:Objective: to investigate the expression of Thyroid transcription-Factor-1- TTF-1 and Ki-67 in non-small cell lung cancerous NSCLC, and to investigate the relationship between them and the clinical parameters of the patients, and to investigate the relationship between the expression of TTF-1 and Ki-67 (proliferative nuclear antigen) in patients with non-small cell lung cancer (NSCLC), and to investigate the relationship between the expression of TTF-1 and Ki-67 in patients with non-small cell lung cancer. Then the relationship between NSCLC and NSCLC is detected by statistical software in order to provide some help for understanding and understanding the characteristics of NSCLC. Methods: selected non-small cell lung cancer (NSCLC) patients hospitalized in Department of Respiratory Medicine and Thoracic surgery from January to June 2015.The source of tissue samples were bronchoscopic biopsy specimens guided by CT and surgical excision specimens. The method of diagnosis was pathological examination (130 cases). The expression of TTF-1 and Ki-67 in these patients was determined by immunohistochemical method to explore the relationship between them and clinical features, and the relationship between TTF-1 and Ki-67. SPSS 17.0. The correlation between TTF-1 and Ki-67 and parameters was measured by chi-square test, and the correlation between TTF-1 and Ki-67 was measured by rank correlation test. Results 1. The positive rate of TTF-1 in NSCLC patients with adenocarcinoma, female and middle and high differentiation stage I-II was higher than that of NSCLC with age, lymph node metastasis, smoking or not. The positive rate of Ki-67 in NSCLC patients with smoking history, low differentiation and lymph node metastasis was higher than that in NSCLC patients with lymph node metastasis. But not related to pathological type, sex, age, stage. There was no significant correlation between the expression of TTF-1 and Ki-67 in NSCLC (r = 0.173, p0.05). Conclusion TTF-1 may play a positive role in the diagnosis of lung adenocarcinoma. In NSCLC patients, the higher the differentiation degree and the earlier the clinical stage, the higher the positive rate of TTF-1 expression. TTF-1 may be involved in the invasion of NSCLC. It may play an important role in the invasion pathway of NSCLC. In NSCLC patients, the lower the differentiation degree, the higher the positive rate of Ki-67 expression. Ki-67 may be involved in the invasion and metastasis of NSCLC. It may play an important role in the infiltration and metastasis of NSCLC.
【学位授予单位】：皖南医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R734.2

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