急性淋巴细胞白血病患者FGFR3表达对循环内皮细胞增殖的影响

发布时间：2018-06-24 00:36

本文选题：急性淋巴细胞白血病 + 成纤维细胞生长因子受体　；参考：《中国病理生理杂志》2017年04期

【摘要】：目的:了解急性淋巴细胞白血病(ALL)患者成纤维细胞生长因子受体3(FGFR3)表达对循环内皮细胞(CECs)增殖的影响。方法:通过RT-PCR法检测44例ALL患者骨髓中FGFR3 mRNA表达,分成FGFR3~+组及FGFR3~-组进行Kaplan-Meier生存分析。利用免疫磁珠结合流式细胞术分离计数CECs,对比两组CECs数量、表面抗原表达、生长曲线及集落生成数的差异。免疫荧光组化染色测定CECs表面FGFR3表达水平。结果:44例核型正常ALL患者中FGFR3 mRNA表达阳性率为43.2%,T-ALL组的FGFR3表达高于B-ALL组(P0.05)。19 d骨髓原始细胞比例≥5%的患者FGFR3表达升高(P0.05)。FGFR3阳性组的总生存率明显低于阴性组(P0.05)。分离出的CECs高表达CD31、CD144、VEGFR-2和CD146,基本不表达CD45。与FGFR3阴性组相比,阳性组CECs数量、CD133的阳性率及集落生成数均增多(P0.05)。体外培养中,FGFR3阳性组与阴性组相比,生长曲线3个时点上的细胞数差异有统计学显著性(P0.05)。19例ALL-FGFR3~+患者CECs表面均能检测到FGFR3表达,阳性率为29.00%±15.71%。结论:致癌基因FGFR3对ALL患者CECs的增殖有促进作用,可能具备了抗肿瘤及抗血管生成的双重靶点身份,可以为今后的分子治疗提供更多的选择。
[Abstract]:Objective: to understand the effect of fibroblast growth factor receptor 3 (FGFR3) expression on the proliferation of circulating endothelial cells (CECs) in patients with acute lymphoblastic leukemia (ALL). Methods: the expression of FGFR3 mRNA in bone marrow of 44 patients with ALL was detected by RT-PCR, and the Kaplan-Meier survival analysis was divided into FGFR3~+ and FGFR3~- groups. The immunomagnetic bead binding flow formula was used. The number of CECs, the expression of surface antigen, the growth curve and the number of colony formation were compared between the two groups of CECs. The expression of FGFR3 expression on the surface of CECs was measured by immunohistochemical staining. Results: the positive rate of FGFR3 mRNA expression was 43.2% in 44 cases of normal ALL patients, and the FGFR3 expression in T-ALL group was higher than that of B-ALL group (P0.05).19 marrow primitive fine bone marrow The total survival rate of FGFR3 expression increased (P0.05).FGFR3 positive group was significantly lower than that of negative group (P0.05). The CECs high expression of CECs expressed CD31, CD144, VEGFR-2 and CD146. The number of CECs in the positive group, the positive rate and the colony formation number in the positive group were increased. Compared with the negative group, there was a significant difference in the number of cells at the 3 time points of the growth curve (P0.05). The expression of FGFR3 was detected on the surface of CECs in.19 cases of ALL-FGFR3~+ patients, and the positive rate was 29% + 15.71%. conclusion: the carcinogenic gene FGFR3 could promote the proliferation of CECs in ALL patients, and may have dual anti tumor and anti angiogenesis. Target status can provide more choices for molecular therapy in the future.
【作者单位】：宁波大学医学院附属鄞州医院血液科;华中科技大学同济医学院同济医院血液科;
【基金】：浙江省自然科学基金资助项目(No.LQ12H08001) 浙江省卫生厅一般研究项目资助(No.201235256) 宁波市自然科学基金资助项目(No.2012A610236) 宁波市医学科技计划资助项目(No.2011B23)
【分类号】：R733.71

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