肝癌细胞中miR-376c的表达及其与高迁移率族蛋白A2的关系

发布时间：2018-06-27 21:59

本文选题：癌 + 肝细胞　；参考：《中国普通外科杂志》2017年07期

【摘要】：目的:探讨肝细胞癌(HCC)中miR-376c的表达情况、临床意义及作用机制。方法:用real-time PCR检测miR-376c在HCC组织与癌旁组织、以及不同HCC细胞系与正常肝细胞中的表达,分析miR-376c的表达与HCC患者临床病理特征及预后的关系;将HCC细胞分别转染miR-376c模拟物和阴性对照组序列后,用Transwell法和划痕实验检测细胞的迁移及侵袭,Western blot检测miR-376c可能的靶点高迁移率族蛋白A2(HMGA2)的表达;用免疫组化检测HCC组织中HMGA2的表达,并分析miR-376c与HMGA2之间的相关性。结果:与癌旁组织或正常肝细胞比较,miR-376c在HCC组织及不同HCC细胞中的表达均明显下调(均P0.05),miR-376c的低表达与门静脉侵犯(P=0.019)、TNM分期(P=0.012)及Edmondson分级(P=0.009)有关;miR-376c低表达的患者3年生存率明显低于其高表达的患者(P=0.0081)。与转染阴性对照序列的HCC细胞比较,转染miR-376c模拟物的HCC细胞的迁移及侵袭数(56.00 vs.26.00;45.33 vs.18.33)明显减少、划痕愈合率明显减低(95.33%vs.60.00%)、HMGA2的表达明显下调(均P0.05);miR-376c高表达的HCC组织中HMGA2表达量明显低于miR-376c低表达的HCC组织(P0.05),HCC组织中miR-376c与HMGA2的表达呈负相关(r=-0.541,P0.01)。结论:miR-376c可能是一种抑癌因子,其在HCC中表达降低,从而削弱了对HMGA2的抑制、促进了HCC细胞的迁移与侵袭。
[Abstract]:Objective: to investigate the expression, clinical significance and mechanism of miR-376c in hepatocellular carcinoma (HCC). Methods: the expression of miR-376c was detected by real-time in HCC tissues and adjacent tissues, as well as in different HCC cell lines and normal hepatocytes. The relationship between miR-376c expression and clinicopathological features and prognosis was analyzed. After the HCC cells were transfected with miR-376c analogue and negative control group respectively, the migration of HCC cells and the expression of miR-376c high mobility group protein A2 (HMGA2) were detected by Transwell method and scratch test. The expression of HMGA2 in HCC was detected by immunohistochemistry and the correlation between miR-376c and HMGA2 was analyzed. Results: the expression of miR-376c in HCC tissues and different HCC cells was significantly down-regulated (P0.05), and the low expression of miR-376c in portal vein invasion (P0. 019) TNM stage (P0. 012) and Edmondson grade (P0. 009) were correlated with the expression of miR-376c in 3 years old patients with low expression of miR-376c (P0. 012) and Edmondson grade (P0. 009). The survival rate was significantly lower than that in the patients with high expression (P0. 0081). The migration and invasion (56.00 vs.26.00 ~ 45.33 vs.18.33) of miR-376c mimetic HCC cells were significantly decreased compared with those of HCC cells transfected with negative control sequence. The expression of HMGA2 in HCC tissues with high expression of miR-376c was significantly lower than that in HCC tissues with low expression of miR-376c (P 0.05). The expression of miR-376c was negatively correlated with HMGA2 expression in HCC tissues (P 0.05). ConclusionMmiR-376c may be a tumor suppressor, and its expression is decreased in HCC, which weakens the inhibition of HMGA2 and promotes the migration and invasion of HCC cells.
【作者单位】：西安交通大学第一附属医院肝胆外科;
【基金】：国家自然科学基金资助项目(81402039) 陕西省自然科学基础研究计划基金资助项目(2016JQ8047)
【分类号】：R735.7

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