胃腺癌患者外周血SDF-1α含量表达检测及与临床病理特征的关系

发布时间：2018-06-28 18:41

本文选题：胃腺癌 + SDF-1α　；参考：《上海交通大学》2015年硕士论文

【摘要】：目的:检测和比较胃腺癌患者血清中SDF-1α蛋白含量的变化及其临床意义。资料与方法:应用酶联免疫吸附测定法检测90例胃腺癌患者以及25例健康志愿者血清中SDF-1α含量,单因素分析其与各项临床病理特征及远处转移(术前常规行胸部及腹部增强CT检测评估患者术前肿瘤转移情况)特征的关系,进而行Logistic回归分析SDF-1α高表达的独立危险因子。Kaplan-Meier法绘制胃腺癌患者生存曲线,采用Log-rank法行单因素生存分析,Cox比例风险回归模型分析影响预后的独立危险因素。结果:胃腺癌患者(n=90)血清SDF-1α含量[(6950.8±1131.3)pg/ml]明显高于健康志愿者组成的正常对照组(n=25)[(5023.7±1103.8)pg/ml,(P=0.036)]。其中发生远处转移的胃腺癌患者(n=12)血清SDF-1α含量[(8251.6±1042.5)pg/ml]显著高于未发生转移患者(n=78)[(6785.3±1025.0)pg/ml,(P0.001)]。胃腺癌患者血清SDF-1α高含量与肿瘤分期较晚(P0.001)、淋巴结转移更为广泛(P=0.018)、肿瘤浸润较深(P0.001)、存在血管侵犯(P0.001)和淋巴管侵犯(P0.001)有关。发生肝转移(P=0.002)和肺转移(P=0.030)的患者外周血SDF-1α含量高于未发生转移的患者。Logistic回归分析提示肿瘤浸润深度是血清SDF-1α高含量的独立危险因子(P=0.027)。外周血SDF-1α高含量患者术后生存时间显著短于低含量患者(P0.001)。Cox比例风险回归模型分析提示肿瘤分期(P=0.009)、血管侵犯程度(P=0.002)及外周血SDF-1α含量(P=0.001)是胃癌患者预后的独立危险因素。结论:胃腺癌患者外周血SDF-1α含量的升高或提示淋巴结、肝及肺转移可能,并与胃癌的预后密切相关。
[Abstract]:Objective: to detect and compare the changes of serum SDF-1 伪 protein in patients with gastric adenocarcinoma and its clinical significance. Materials and methods: the serum levels of SDF-1 伪 in 90 patients with gastric adenocarcinoma and 25 healthy volunteers were detected by enzyme-linked immunosorbent assay (Elisa). Univariate analysis was used to analyze the relationship between the clinicopathological features and distant metastasis (preoperative routine chest and abdominal enhanced CT examination to evaluate preoperative tumor metastasis). Furthermore, the survival curve of gastric adenocarcinoma patients was drawn by logistic regression analysis with independent risk factor. Kaplan-Meier method. Single factor survival analysis was performed by Log-rank method and Cox proportional risk regression model was used to analyze the independent risk factors affecting prognosis. Results: the level of serum SDF-1 伪 in patients with gastric adenocarcinoma [(6950.8 卤1131.3) pg/ml] was significantly higher than that in healthy volunteers (n = 25) [(5023.7 卤1103.8) PG / ml, (P0. 036)]. The level of serum SDF-1 伪 in patients with distant metastasis (NM12) [(8251.6 卤1042.5) pg/ml] was significantly higher than that in patients without metastasis (n = 78) [(6785.3 卤1025.0) PG / ml, (P0.001)]. The high level of serum SDF-1 伪 in patients with gastric adenocarcinoma was related to the later stage (P0.001), the more extensive lymph node metastasis (P0. 018), the deeper invasion of tumor (P0. 001), the presence of vascular invasion (P0. 001) and lymphatic invasion (P0. 001). The level of SDF-1 伪 in peripheral blood of patients with liver metastasis (P0. 002) and pulmonary metastasis (P0. 030) was higher than that of patients without metastasis. Logistic regression analysis showed that the depth of tumor invasion was an independent risk factor for the high level of serum SDF-1 伪 (P0. 027). The survival time of patients with high level of peripheral blood SDF-1 伪 was significantly shorter than that of patients with low level of SDF-1 伪 (P0.001). Cox proportional risk regression model showed that tumor stage (P0. 009), degree of vascular invasion (P0. 002) and peripheral blood SDF-1 伪 content (P0. 001) were independent risk factors for prognosis of gastric cancer patients. Conclusion: the increase of SDF-1 伪 level in peripheral blood of patients with gastric adenocarcinoma suggests that lymph nodes, liver and lung metastasis may be associated with the prognosis of gastric cancer.
【学位授予单位】：上海交通大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R735.2

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