HER2抑制上皮钙黏素表达促进人乳腺上皮细胞迁移

发布时间：2018-06-30 10:41

本文选题：乳腺上皮细胞 + 人表皮生长因子受体(HER)　；参考：《细胞与分子免疫学杂志》2017年06期

【摘要】：目的研究原癌基因人表皮生长因子受体2(HER2)对MCF10A人乳腺上皮细胞形态和迁移能力的影响及机制。方法通过慢病毒包装及感染MCF10A人乳腺上皮细胞,建立HER2过表达细胞,用TranswellTM法研究细胞迁移能力变化,结合Western blot法、实时定量PCR和免疫荧光染色检测细胞中上皮钙黏素(E-cadherin)的表达。结果过表达HER2的乳腺上皮细胞呈分散型生长,细胞迁移能力增强,E-cadherin蛋白表达受到抑制,细胞膜上E-cadherin表达也显著降低,重新过表达E-cadherin能抑制HER2诱导的细胞迁移和分散。结论 HER2能通过抑制E-cadherin促进人乳腺上皮细胞迁移。
[Abstract]:Objective to investigate the effect and mechanism of proto-oncogene human epidermal growth factor receptor 2 (HER2) on the morphology and migration of MCF10A human mammary epithelial cells. Methods HER2 overexpression cells were established by packaging and infecting MCF10A human mammary epithelial cells by lentivirus. Transwell TM method was used to study the changes of cell migration ability. The expression of E-cadherin was detected by real-time quantitative PCR and immunofluorescence staining with Western blot. Results the overexpression of HER2 in breast epithelial cells was characterized by dispersive growth. The expression of E-cadherin protein was inhibited and the expression of E-cadherin was decreased significantly. E-cadherin reexpression could inhibit the migration and dispersion induced by HER2. Conclusion Her2 can promote the migration of human mammary epithelial cells by inhibiting E-cadherin.
【作者单位】：长江师范学院生命科学与技术学院;
【基金】：重庆市教委科研基金(KJ1601207)
【分类号】：R737.9

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