急性早幼粒细胞白血病细胞来源微粒在凝血紊乱中的作用研究

发布时间：2018-07-02 07:08

本文选题：急性早幼粒细胞白血病 + 微粒　；参考：《中国实验血液学杂志》2017年03期

【摘要】：目的:评估急性早幼粒细胞白血病(acute promyelocytic leukemia,APL)细胞来源微粒(microparticle,MP)及其携带的组织因子(tissue factor,TF)在患者高凝状态中的作用,以及化疗/分化治疗药物对MP促凝血活性(procoagulant activity,PCA)的影响。方法:提取5例APL患者和年龄性别相匹配的5例缺铁性贫血患者(对照)的骨髓单个核细胞(MNC),体外培养48 h,收集含MP细胞培养液和去MP细胞培养液,从一定体积的含MP细胞培养液中提取MP。采用ELISA法检测MP上TF的表达,应用凝血酶生成实验检测含MP细胞培养液和去MP细胞培养液的PCA,利用人TF特异抗体检测TF在MP相关PCA中的作用。测定全反式维甲酸(ATRA)、三氧化二砷(ATO)和柔红霉素(DNR)处理APL细胞48 h后MP的PCA。结果:对照者骨髓MNC释放的MP几乎无TF表达,而APL细胞来源的MP明显表达TF。无论是APL患者骨髓MNC还是对照者骨髓MNC,释放的MP均具有明显的PCA,但与对照者骨髓MNC释放的MP相比,APL患者骨髓MNC释放的MP的PCA更高。TF在对照者骨髓MNC释放的MP的PCA中几乎无作用,而在APL患者骨髓MNC细胞来源的MP的PCA中发挥重要作用。DNR处理的APL患者骨髓MNC能使其释放的MP的PCA增高,而ATO和ATRA的作用则完全相反。结论:APL患者骨髓MNC来源的微粒具有明显的PCA,而TF在这一活性中发挥着重要作用。化疗药物DNR可增强APL患者骨髓MNC释放的MP的PCA明显,而分化治疗药物ATRA和ATO则可降低这一活性
[Abstract]:Aim: to evaluate the role of microparticle MP and its tissue factor-TF in hypercoagulability in patients with acute promyelocytic leukemia (acute promyelocytic leukemia) and the effect of chemotherapy / differentiation therapy on procoagulant activityPCA in patients with acute promyelocytic leukemia. Methods: bone marrow mononuclear cells (MNC) were extracted from 5 APL patients and 5 age-sex matched iron deficiency anemia patients (control group). MP3 was extracted from a certain volume of MP cell culture medium. The expression of TF on MP was detected by Elisa, the PCAs containing and without MP cells were detected by thrombin production assay, and the role of TF in MP related PCA was detected by human TF specific antibody. APL cells were treated with all-trans retinoic acid (ATRA), arsenic trioxide (ATO) and daunorubicin (DNR) for 48 h. Results: MP released from bone marrow MNC of control group showed little TF expression, while MP from APL cells expressed TF. No matter the bone marrow MNC of APL patients or the control group, the MP released by bone marrow showed significant PCA.However, compared with the MP released by bone marrow MNC of control group, the PCA of MP released by bone marrow MNC of APL patients was higher. TF had little effect on the PCA of MP released by bone marrow MNC in control group. MNC of bone marrow of APL patients treated with DNR could increase the PCA of MP, but the effects of ATO and ATRA were opposite. Conclusion the microparticles derived from bone marrow MNC of the patients with Wight APL have obvious PCAs, and TF plays an important role in this activity. The chemotherapeutic drug DNR could enhance the PCA of MP released by bone marrow MNC in APL patients, but ATRA and ATO could decrease the activity.
【作者单位】：哈尔滨医科大学附属第一医院血液肿瘤中心;哈尔滨医科大学附属第一医院中心实验室;
【基金】：黑龙江省教育厅科学技术研究项目(12541345)
【分类号】：R733.71

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