HBV-X突变与TGF-β1致肝细胞肝癌相关性分析

发布时间：2018-07-02 11:10

本文选题：HBV-X基因 + 点突变　；参考：《重庆医科大学》2017年硕士论文

【摘要】：目的:探讨乙肝病毒X区基因变异情况与慢性HBV感染患者血清中TGF-β1表达致肝细胞肝癌间的相关性。方法:收集2015年1月至2016年10月期间重庆医科大学附属第二医院的慢性HBV患者血清,总共105例,其中包括肝炎组35例,肝硬化组35例,肝癌组35例,同时收集患者的基本资料、两对半定量等实验室检查结果。采用聚合酶链反应(PCR)方法扩增HBV-X基因序列,并对PCR产物进行DNA测序,将测序结果与已知HBV-X基因序列进行对比分析,得出变异位点。酶联免疫吸附测定法(ELISA)检测患者血清中TGF-β1浓度。将所得的结果进行卡方检验、单因素方差分析及相关性分析等方法处理数据。结果:TGF-β1浓度在肝癌组表达明显高于非肝癌组(34.4±29.9 vs11.4±16.8和10.1±12.2,P0.05),差异具有统计学意义。肝病患者血清中常见的突变位点依次为:A1762T/G1764A、T1719C、G1635A、A1605G、C1653T、T1753G,其中A1605G、A1762T/G1764A、T1753G突变率在肝癌组明显高于非肝癌组,差异具有统计学意义(P0.05)。A1605G、T1719C位点突变与血清中TGF-β1浓度有相关性(r=0.513,P0.01、r=0.277,P0.01)。进一步分析发现,在肝癌组中仅A1605G位点突变与TGF-β1浓度呈正相关(r=0.518,P0.05)。结论:HBV-X基因中的A1605G位点突变与TGF-β1表达呈正相关,这可能参与了HCC的发生发展进程。
[Abstract]:Objective: to investigate the relationship between the mutation of hepatitis B virus X region gene and the expression of TGF- 尾 1 in serum of patients with chronic HBV infection. Methods: from January 2015 to October 2016, 105 patients with chronic HBV were collected from the second affiliated Hospital of Chongqing Medical University, including 35 patients with hepatitis, 35 patients with liver cirrhosis and 35 patients with liver cancer. Two pairs of semi-quantitative laboratory results. The HBV-X gene sequence was amplified by polymerase chain reaction (PCR), and the DNA sequence of the PCR product was sequenced. The results were compared with the known HBV-X gene sequence, and the mutation sites were obtained. The concentration of TGF- 尾 1 in serum was detected by enzyme-linked immunosorbent assay (Elisa). The results were processed by chi-square test, single factor analysis of variance and correlation analysis. Results the expression of TGF- 尾 1 in HCC group was significantly higher than that in non-HCC group (34.4 卤29.9 vs11.4 卤16.8,10.1 卤12.2 P 0.05), and the difference was statistically significant. The common mutation sites in the serum of liver disease patients were as follows: 1. A1762T / G1764AnT1719CG1635AA1605GC1653T1653T1753G, in which the mutation rate of A1605GN A1762T / G1764AN T1753G was significantly higher in HCC group than in non-HCC group, the difference was statistically significant (P0.05) .A1605GT1719C mutation was correlated with serum TGF- 尾 _ 1 concentration (r0.513P 0.01r277P0.01). The results showed that the mutation of T1753G in liver cancer group was significantly higher than that in non-hepatoma group (P0.05). There was a significant correlation between TGF- 尾 _ 1 mutation and serum TGF- 尾 _ 1 concentration (r 0.513 P 0.01r 277P0.01). Further analysis showed that only A1605G mutation was positively correlated with TGF- 尾 1 concentration in HCC group (r = 0.518, P 0.05). Conclusion the A1605G mutation in the 1: HBV-X gene is positively correlated with the expression of TGF- 尾 1, which may be involved in the development of HCC.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.7

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