细胞自噬在头颈部鳞状细胞癌侵袭转移中的作用

发布时间：2018-07-03 12:58

本文选题：自噬 + 转化生长因子β　；参考：《临床耳鼻咽喉头颈外科杂志》2017年10期

【摘要】：目的:探讨自噬在TGF-β1调控EMT诱导头颈鳞癌侵袭转移中的作用。方法:不同浓度及时间点TGF-β1构建头颈鳞癌上皮-间质转化(EMT)模型,Western blot检测自噬相关蛋白LC3水平;自噬抑制剂氯喹(CQ)抑制自噬后,Western blot检测上皮细胞标志物钙黏蛋白、间质细胞标志物波形蛋白及LC3蛋白的表达;划痕、Transwell侵袭小室实验检测自噬对头颈鳞癌侵袭、转移的影响。结果:不同浓度及时间点TGF-β1诱导EMT的同时,诱导发生自噬,且随着TGF-β1浓度的增加及时间的延长,自噬随之增强;自噬被抑制后,E-cadherin表达上调、Vimentin表达下调,细胞迁移及侵袭潜能较对照组明显减弱。结论:TGF-β1诱导头颈鳞癌发生EMT的同时伴随自噬;自噬在TGF-β1调控头颈鳞癌EMT的过程中,能增强其侵袭、转移能力。
[Abstract]:Objective: To investigate the role of autophagy in the regulation of EMT induced invasion and metastasis of head and neck squamous cell carcinoma by TGF- beta 1. Methods: TGF- beta 1 at different concentrations and time points was used to construct the epithelial mesenchymal transition (EMT) model of head and neck squamous cell carcinoma, and Western blot was used to detect the level of autophagy associated protein LC3; the autophagic inhibitor chloroquine (CQ) inhibited autophagy, and Western blot was used to detect the epithelial cell marker calcium. The expression of visco, stromal cell marker, vimentin and LC3 protein; scratch, Transwell invasion laboratory test to detect the effect of autophagy on the invasion and metastasis of head and neck squamous cell carcinoma. Results: TGF- beta 1 induced EMT at different concentrations and time points, induced autophagy, and autophagy increased with the increase of TGF- beta 1 concentration and time. After inhibition, E-cadherin expression was up, Vimentin expression was down, cell migration and invasion potential decreased significantly compared with the control group. Conclusion: TGF- beta 1 induced EMT in head and neck squamous cell carcinoma with autophagy, and autophagy can enhance its invasion and metastasis in the process of TGF- beta 1 in the regulation of head and neck squamous cell carcinoma.
【作者单位】：中南大学湘雅医院耳鼻咽喉头颈外科湖南省耳鼻咽喉重大疾病研究重点实验室;中南大学湘雅二医院耳鼻咽喉头颈外科;中南大学湘雅医学院附属肿瘤医院湖南省肿瘤医院头颈外科;
【基金】：国家自然科学基金(No:81472696);国家自然科学基金(No:81602389) 湖南省自然科学基金(No:2017JJ3456) 中南大学中央高校基本科研业务费专项资金资助(No:2016zzts525)
【分类号】：R739.91

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