MiR-34a在牙龈鳞状细胞癌组织中的表达及其临床意义

发布时间：2018-07-12 17:01

本文选题：microRNA + 34a　；参考：《吉林大学》2017年硕士论文

【摘要】：目的:通过检测牙龈鳞状细胞癌(gingival squamous cell carcinoma,GSCC)及癌旁非瘤组织中小分子RNA 34a(micro RNA 34a,miR-34a)的表达水平,探讨miR-34a与GSCC发病部位、大小、恶性程度以及患者的年龄和性别等临床病理特征之间的关系。方法:在患者知情同意下,收集2014-2016年吉林大学口腔医学院颌面外科30例确诊为GSCC患者的手术切除肿瘤及相应癌旁非瘤组织。采用qRT-PCR法检测GSCC及癌旁非瘤组织中miR-34a的表达水平。结果:与癌旁非瘤组织相比,GSCC组织中miR-34a的表达水平明显下降(0.47±0.26,P=0.004);GSCC患者肿瘤组织中miR-34a的表达水平与年龄、性别、所在部位及有无淋巴结转移等无关联(P0.05),但与肿瘤组织的大小及恶性程度有关联(P0.05)。结论:1.通过qRT-PCR技术检测,miR-34a在GSCC患者肿瘤组织中的表达较癌旁非瘤组织明显下调,提示可能与GSCC的发生、发展密切相关。2.结合临床病理资料,在GSCC患者的肿瘤组织中,miR-34a相对表达水平降低,且与肿瘤体积,分化程度关系密切(P0.05),提示可进一步作为临床上GSCC的重要诊断指标,并对患者术后肿瘤复发、死亡等预后作出更准确预测,为进一步个体化,精准的癌症治疗提供依据。
[Abstract]:Objective: to detect the expression of small molecule RNA34a (micro RNA34a) in (gingival squamous cell carcinomas of gingival squamous cell carcinoma (GSCC) and its adjacent non-tumor tissues, and to investigate the location and size of miR-34a and GSCC. The relationship between malignancy and clinicopathological features such as age and sex. Methods: 30 patients with GSCC diagnosed as GSCC from 2014 to 2016 were collected with informed consent from Jilin University School of Stomatology. The expression of miR-34a in GSCC and noncancerous tissues was detected by qRT-PCR. Results: the expression level of miR-34a in GSCC was significantly decreased (0.47 卤0.26 P0. 004) compared with that in non-cancerous tissues adjacent to cancer. The expression level of miR-34a in GSCC patients was significantly lower than that in patients with GSCC, and the expression level of miR-34a in GSCC was significantly lower than that in patients with GSCC. There was no correlation between the location and lymph node metastasis (P0.05), but there was a correlation with the size of tumor tissue and the degree of malignancy (P0.05). Conclusion 1. The expression of miR-34a in GSCC patients was down-regulated by qRT-PCR, which suggested that it might be closely related to the occurrence and development of GSCC. In combination with clinicopathological data, the relative expression of miR-34a was decreased in GSCC patients, and was closely related to tumor volume and differentiation (P0.05), which suggested that it could be further used as an important diagnostic index for GSCC and the recurrence of GSCC after operation. The prognosis of death is predicted more accurately, which provides the basis for further individualized and accurate cancer treatment.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R739.8

【相似文献】