miRNA基因单核苷酸多态性与非小细胞肺癌易感性的关联研究

发布时间：2018-07-16 07:48

【摘要】：目的探讨微小RNA(micro RNA,miRNA)基因单核苷酸多态性(single nucleotide polymorphism,SNP)与非小细胞肺癌(non-small cell lung cancer,NSCLC)发生风险的关系。方法通过生物信息学方法筛选出13个位于miRNA基因序列上的潜在功能性的SNPs位点。采用病例-对照研究设计,纳入新发NSCLC患者626例,对照为736例同期门诊体检人群。提取研究对象外周血细胞基因组DNA,采用SNPscan~(TM)对筛选的13个SNPs位点进行基因分型,多因素Logistic回归分析SNPs与肺癌发生风险的关联性和关联强度。结果发现3个SNPs,即rs62085660(位于miRNA AC145343.1)、rs11597888(位于miRNA AL391839.1)和rs16867808(位于miRNA AL021918.2)与NSCLC发生风险显著相关,但此种关联只限于特定的亚组人群。rs62085660(C/G)位点与肺鳞癌的发生风险相关(加性模型,校正OR=0.79,95%CI=0.62~1.00,P=0.049;显性模型,校正OR=0.72,95%CI=0.53~0.98,P=0.035)。rs11597888(G/A)和rs16867808(T/C)位点只在吸烟人群中与肺癌易感性相关(rs11597888:显性模型,校正OR=1.42,95%CI=1.03~1.96,P=0.033;rs16867808:加性模型,校正OR=0.58,95%CI=0.40~0.84,P=0.004,显性模型,校正OR=0.53,95%CI=0.35~0.81,P=0.004)。结论 rs62085660位点与肺鳞癌发生风险相关,rs11597888位点和rs16867808位点与吸烟相关性肺癌发生风险相关。
[Abstract]:Objective to investigate the relationship between the single nucleotide polymorphism (single nucleotide polymorphisms (SNPs) of the micro RNA gene and the risk of non-small cell lung cancer (NSCLC). Methods Thirteen potential functional SNPs loci located on miRNA gene sequences were screened by bioinformatics. 626 newly diagnosed NSCLC patients were enrolled in the case-control study design, and 736 outpatient physical examination subjects were included in the control group. The genomic DNA of peripheral blood cells was extracted and the 13 SNPs loci were genotyped by SNPscanTM. Multivariate logistic regression analysis showed that SNPs were associated with the risk of lung cancer. The results showed that three SNPs, rs62085660 (located in miRNA AC145343.1), rs11597888 (located in miRNA AL391839.1) and rs16867808 (miRNAAL021918.2) were significantly associated with the risk of occurrence of lung squamous cell carcinoma, but the association was limited to the specific subgroup population. Rs62085660 (Crs62085660) site was associated with the risk of lung squamous cell carcinoma (additive model, OR0.7995CI0.62T 1.00P0.049; dominant model). Rs11597888 (G / A) and rs16867808 (T / C) were only associated with lung cancer susceptibility in smokers (rs115978888: dominant model, OR 1.031.96P0.033rs16867808: additive model, OR0.5895CII 0.400.84P0. 004, dominant model, OR0.5395CI0.350.381P0. 004). Conclusion rs62085660 locus is associated with the risk of lung squamous cell carcinoma. Rs11597888 locus and rs16867808 locus are associated with the risk of lung cancer associated with smoking.
【作者单位】：第三军医大学军事预防医学院军队流行病学教研室;第三军医大学新桥医院胸外科;第三军医大学新桥医院全军呼吸内科研究所全军呼吸病研究重点实验室;
【基金】：国家自然科学基金面上项目(81171903,81472190,81672316) 重庆市自然科学基金重点项目(CSTC2015jcyjBX0110)~~
【分类号】：R734.2

【相似文献】