毛细管电泳技术在T细胞淋巴瘤诊断中的应用
发布时间：2018-07-16 18:27
【摘要】：目的淋巴瘤的发生被认为是B或T淋巴细胞被阻断在其分化过程中某一阶段而造成淋巴细胞的克隆性增生所致。多数淋巴系统增生性疾病通过组织形态学和免疫表型分析能够明确诊断,但对于T细胞淋巴瘤(T cell Lymphoma,TCL)的诊断,仅凭形态学与免疫表型分析难以明确。本研究旨在探讨毛细管电泳技术在TCL诊断中的应用价值。方法回顾性分析2014-01-04-2015-06-30齐鲁医学检验所经形态学和免疫组织化学诊断的TCL和不能明确诊断的淋巴组织增生性病变共63例,利用毛细管电泳基因片段分析技术分析基因重排产物的克隆性。结果 25例TCL中,T细胞受体-G(T-cell receptor G,TCRG)、TCRB、TCRD基因重排的检出率分别为88.0%(22/25)、72.0%(18/25)和20.0%(5/25),三者联合检出率为96.0%(24/25)。38例未明确诊断的淋巴组织增生性病变中,21例为多克隆性增生。结论毛细管电泳基因片段分析对TCL的诊断能够提供遗传学的支持,并且对形态学及免疫组化不典型或疑难病例可以提供诊断方向,利于TCL的病理诊断及鉴别诊断。
[Abstract]:Objective Lymphoma is thought to be due to the clonal proliferation of lymphocytes due to the blocking of B or T lymphocytes at a certain stage of their differentiation. Most lymphoproliferative diseases can be diagnosed by histomorphologic and immunophenotypic analysis, but the diagnosis of T cell lymphoma (T cell lymphoma) is difficult to be determined by morphological and immunophenotypic analysis alone. The purpose of this study was to explore the value of capillary electrophoresis in the diagnosis of TCL. Methods 63 cases of lymphoproliferative lesions diagnosed by morphology and immunohistochemistry in Qilu Medical examination Institute 2014-01-04-2015-06-30 were retrospectively analyzed. The clonality of gene rearrangement products was analyzed by capillary electrophoresis gene fragment analysis. Results the positive rates of T-cell receptor -G (T-cell receptor) TCRD gene rearrangement were 88.0% (22 / 25) 72.0% (18 / 25) and 20.0% (5 / 25), respectively. The combined detection rate was 96.0% (24 / 25) .38 cases of undiagnosed lymphoproliferative lesions were polyclonal hyperplasia. Conclusion Capillary electrophoresis gene fragment analysis can provide genetic support for the diagnosis of TCL, and can provide diagnostic direction for atypical or difficult cases of morphology and immunohistochemistry, which is helpful to the pathological diagnosis and differential diagnosis of TCL.
【作者单位】： 山东省交通医院病理科;天津医科大学附属肿瘤医院病理科;齐鲁医学检验所病理科;
【基金】：山东省交通运输科技计划(2013B24)
【分类号】：R733.1
，
本文编号：2127260
[Abstract]:Objective Lymphoma is thought to be due to the clonal proliferation of lymphocytes due to the blocking of B or T lymphocytes at a certain stage of their differentiation. Most lymphoproliferative diseases can be diagnosed by histomorphologic and immunophenotypic analysis, but the diagnosis of T cell lymphoma (T cell lymphoma) is difficult to be determined by morphological and immunophenotypic analysis alone. The purpose of this study was to explore the value of capillary electrophoresis in the diagnosis of TCL. Methods 63 cases of lymphoproliferative lesions diagnosed by morphology and immunohistochemistry in Qilu Medical examination Institute 2014-01-04-2015-06-30 were retrospectively analyzed. The clonality of gene rearrangement products was analyzed by capillary electrophoresis gene fragment analysis. Results the positive rates of T-cell receptor -G (T-cell receptor) TCRD gene rearrangement were 88.0% (22 / 25) 72.0% (18 / 25) and 20.0% (5 / 25), respectively. The combined detection rate was 96.0% (24 / 25) .38 cases of undiagnosed lymphoproliferative lesions were polyclonal hyperplasia. Conclusion Capillary electrophoresis gene fragment analysis can provide genetic support for the diagnosis of TCL, and can provide diagnostic direction for atypical or difficult cases of morphology and immunohistochemistry, which is helpful to the pathological diagnosis and differential diagnosis of TCL.
【作者单位】： 山东省交通医院病理科;天津医科大学附属肿瘤医院病理科;齐鲁医学检验所病理科;
【基金】：山东省交通运输科技计划(2013B24)
【分类号】：R733.1
，
本文编号：2127260
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