金纳米笼在近红外光激发下诱导产生活性氧的研究

发布时间：2018-07-17 08:21

【摘要】：目的研究纳米金笼光热诱导过氧化氢(H_2O_2)增强活性氧(ROS)的情况,分析ROS种类及其产量与H_2O_2浓度的关系,为金纳米笼在光热/光动力治疗领域的深入研究提供理论依据。方法利用808 nm近红外激光照射不同浓度的H_2O_2与金纳米笼的混合溶液后,通过酶标仪和ROS荧光探针检测产生ROS的种类和含量。结果金纳米笼在近红外光激发下,由于表面等离子体共振效应(SPR),不仅能产生过热效应,还能诱导H_2O_2产生更多的ROS(种类主要为单线态氧和羟基自由基);并且随着H_2O_2浓度的增加,单线态氧和羟基自由基的含量也会增加。结论金纳米笼在近红外光激发下能诱导H_2O_2产生单线态氧和羟基自由基等ROS,且与H_2O_2浓度成正相关。
[Abstract]:Aim to study the enhancement of reactive oxygen species (Ros) by H _ 2O _ 2 induced by photothermal hydrogen peroxide (H _ 2O _ 2) in gold nanocage, and to analyze the relationship between Ros species and H _ 2O _ 2 concentration in order to provide a theoretical basis for the further study of gold nanoparticles in the field of photothermal / photodynamic therapy. Methods the mixed solution of H _ 2O _ 2 and gold nanocage was irradiated with 808 nm near infrared laser. The species and content of Ros were detected by enzyme scale and Ros fluorescence probe. Results due to the surface plasmon resonance (SPR) effect, gold nanocages can not only produce overheating effect, but also induce more Ros (mainly singlet oxygen and hydroxyl radical) induced by H _ 2O _ 2, and with the increase of H _ 2O _ 2 concentration, the gold nanocage can not only produce superheated effect, but also induce H _ 2O _ 2 to produce more Ros (mainly singlet oxygen and hydroxyl radical). The content of singlet oxygen and hydroxyl radical will also increase. Conclusion Gold nanocage can induce H _ 2O _ 2 to produce singlet oxygen and hydroxyl radical under near-infrared excitation, which is positively related to the concentration of H _ 2O _ 2.
【作者单位】：广东医科大学药学院;
【基金】：国家自然科学青年科学基金资助项目(81401520) 广东省中医药局面上科研资助项目(20171154) 东莞市社会科技发展资助项目(2015108101019) 广东医科大学博士科研启动课题资助(2XB14015)
【分类号】：O657.3;R730.5

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