胱硫醚-γ-裂解酶在肺癌中表达和临床意义研究
发布时间：2018-07-23 19:25
【摘要】：目的:本项研究主要利用免疫组织化学(Immunohistochemistry)和蛋白质印迹(Western Blot)两种实验方法来检测内源性硫化氢合成酶胱硫醚-γ-裂解酶(CSE)在行肺癌根治性切除术后并经过病理验证的肺癌组织与癌旁正常组织(此癌旁正常组织距离癌组织至少5cm以上)两组病例标本中的表达水平及对其临床意义进行研究。方法:本项实验研究按照选取标准严格选取了从2010年1月?2012年1月期间在青岛大学附属青岛市市立医院医院行肺癌根治手术后的肺癌病理标本125例和距离癌组织5 cm以上癌旁正常肺组织标本125例(其中病理组织标本的结果已经由青岛大学附属青岛市市立医院医院病理科阅片后明确诊断)。所有选取病例手术前均未实行化学药物和放射治疗。应用免疫组化技术和Western Blot检测两种实验技术检测胱硫醚-γ-裂解酶在125例肺癌组织及相应癌旁正常黏膜的表达情况,并通过门诊回访或者电话咨询进行随访。利用SPSS 13.0统计学软件分析本实验研究相关资料:肺癌组织胱硫醚-γ-裂解酶的表达和其在癌旁正常肺癌组织中的表达存在明显差异,胱硫醚-γ-裂解酶的表达情况与肺癌患者病理及临床因素之间的关系。采用Kaplan-Meier法进行生存分析并绘制生存曲线图,用Log-rank检验其生存差异性。其中P0.05时表示差异存在统计学意义。结果:(1)胱硫醚-γ-裂解酶在肺癌组织明显表达,阳性表达主要在表达细胞质中,呈棕黄色。胱硫醚-γ-裂解酶在肺癌组织和癌旁正常肺癌组织中的表达水平存在差异:肺癌组织中胱硫醚-γ-裂解酶的表达水平显著高于其在癌旁正常组织的表达,两者之间表达水平差异有统计学意义(P0.05)。同时蛋白质印迹Western Blot法检测再次证明肺癌组织中胱硫醚-γ-裂解酶的表达水平明显比肺癌旁正常组织中的表达水平高。(2)在肺癌组织中胱硫醚-γ-裂解酶的表达水平与病理及临床因素之间的关系和肺癌患者5年生存率均存在差异:胱硫醚-γ-裂解酶的表达情况与TNM分期情况、周围淋巴结发生转移与否、肿瘤大小、肿瘤分化程度有关(P0.05);胱硫醚-γ-裂解酶低表达的肺癌患者5年生存率明显高于胱硫醚-γ-裂解酶高表达的肺癌患者(P0.05)。结论:胱硫醚-γ-裂解酶在肺癌组织中呈现高水平表达,可能在肺癌的发生、浸润转移等演变过程中发挥重要作用,胱硫醚-γ-裂解酶在肺癌细胞中的表达及胱硫醚-γ-裂解酶对肺癌细胞增殖和凋亡的影响将有助于了解胱硫醚-γ-裂解酶对肺癌肿瘤中的生物学效应,可为以后胱硫醚-γ-裂解酶和肺癌方面的更深层次的研究提供一定程度的研究思路和理论基础,同时对分子致病机制的深入研究可为肺癌的预防与治疗提供一些策略和手段,因此以胱硫醚-γ-裂解酶为靶点的研究新思路将可作为评估肺癌早期发生及预后情况的参考指标。
[Abstract]:Objective: to detect endogenous hydrogen sulfide synthase cystathithione- 纬 -lyase (CSE) by immunohistochemical (Immunohistochemistry) and Western blot (Western Blot) after radical resection of lung cancer. The expression level and clinical significance of the two groups of lung cancer tissues and adjacent normal tissues (at least above 5cm) were studied. Methods: according to the selection criteria, 125 lung cancer specimens from January 2010 to January 2012 after radical lung cancer surgery were selected from Qingdao City Hospital affiliated to Qingdao University. 125 cases of normal lung tissue above 5 cm (the results of pathological tissue were diagnosed by the pathology department of Qingdao Municipal Hospital affiliated to Qingdao University). All the selected cases were not treated with chemotherapy and radiotherapy before operation. Immunohistochemical and Western Blot techniques were used to detect the expression of cystein-纬 -lyase in 125 cases of lung cancer and its adjacent normal mucosa, and followed up by out-patient visit or telephone consultation. The data of this study were analyzed by SPSS 13.0 software. The expression of cystathithione- 纬 -lyase in lung cancer tissues was significantly different from that in adjacent normal lung cancer tissues. Relationship between expression of cystathithione-纬-lyase and pathological and clinical factors in patients with lung cancer. Kaplan-Meier method was used to analyze the survival and draw the survival curve, and Log-rank was used to test its survival difference. Among them, P0.05 time indicates that the difference is statistically significant. Results: (1) cystathithione- 纬 -lyase was obviously expressed in lung cancer tissues, and the positive expression was mainly in the cytoplasm of lung cancer. The expression level of cystathithione- 纬 -lyase was significantly higher in lung cancer tissues than in adjacent normal tissues, and the expression level of cystathithione- 纬 -lyase in lung cancer tissues was significantly higher than that in adjacent normal tissues. There was significant difference in expression level between the two groups (P0.05). At the same time, Western blot Western Blot assay showed that the expression level of cystathithione- 纬 -lyase in lung cancer tissues was significantly higher than that in normal tissues adjacent to lung cancer. (2) the expression level of cystathion- 纬 -lyase in lung cancer tissues was similar to that in lung cancer tissues. The relationship between pathological and clinical factors and the 5-year survival rate of lung cancer patients were different: the expression of cystathion-gamma lyase and the TNM stage. The 5-year survival rate of lung cancer patients with low expression of cystathion- 纬-lyase was significantly higher than that of lung cancer patients with high expression of cystathion- 纬-lyase (P0.05). Conclusion: cystathithione- 纬 -lyase is highly expressed in lung cancer, which may play an important role in the development of lung cancer, invasion and metastasis. The expression of cystathithione- 纬 -lyase in lung cancer cells and the effect of cystathion- 纬 -lyase on proliferation and apoptosis of lung cancer cells will be helpful to understand the biological effects of cystathion- 纬 -lyase on lung cancer tumors. It may provide a certain degree of theoretical basis for the further research on cystathion-gamma lyase and lung cancer, and provide some strategies and means for the prevention and treatment of lung cancer. Therefore, the new idea of cystathithione- 纬 -lyase as a target may be used as a reference index to evaluate the early occurrence and prognosis of lung cancer.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R734.2
本文编号：2140413
[Abstract]:Objective: to detect endogenous hydrogen sulfide synthase cystathithione- 纬 -lyase (CSE) by immunohistochemical (Immunohistochemistry) and Western blot (Western Blot) after radical resection of lung cancer. The expression level and clinical significance of the two groups of lung cancer tissues and adjacent normal tissues (at least above 5cm) were studied. Methods: according to the selection criteria, 125 lung cancer specimens from January 2010 to January 2012 after radical lung cancer surgery were selected from Qingdao City Hospital affiliated to Qingdao University. 125 cases of normal lung tissue above 5 cm (the results of pathological tissue were diagnosed by the pathology department of Qingdao Municipal Hospital affiliated to Qingdao University). All the selected cases were not treated with chemotherapy and radiotherapy before operation. Immunohistochemical and Western Blot techniques were used to detect the expression of cystein-纬 -lyase in 125 cases of lung cancer and its adjacent normal mucosa, and followed up by out-patient visit or telephone consultation. The data of this study were analyzed by SPSS 13.0 software. The expression of cystathithione- 纬 -lyase in lung cancer tissues was significantly different from that in adjacent normal lung cancer tissues. Relationship between expression of cystathithione-纬-lyase and pathological and clinical factors in patients with lung cancer. Kaplan-Meier method was used to analyze the survival and draw the survival curve, and Log-rank was used to test its survival difference. Among them, P0.05 time indicates that the difference is statistically significant. Results: (1) cystathithione- 纬 -lyase was obviously expressed in lung cancer tissues, and the positive expression was mainly in the cytoplasm of lung cancer. The expression level of cystathithione- 纬 -lyase was significantly higher in lung cancer tissues than in adjacent normal tissues, and the expression level of cystathithione- 纬 -lyase in lung cancer tissues was significantly higher than that in adjacent normal tissues. There was significant difference in expression level between the two groups (P0.05). At the same time, Western blot Western Blot assay showed that the expression level of cystathithione- 纬 -lyase in lung cancer tissues was significantly higher than that in normal tissues adjacent to lung cancer. (2) the expression level of cystathion- 纬 -lyase in lung cancer tissues was similar to that in lung cancer tissues. The relationship between pathological and clinical factors and the 5-year survival rate of lung cancer patients were different: the expression of cystathion-gamma lyase and the TNM stage. The 5-year survival rate of lung cancer patients with low expression of cystathion- 纬-lyase was significantly higher than that of lung cancer patients with high expression of cystathion- 纬-lyase (P0.05). Conclusion: cystathithione- 纬 -lyase is highly expressed in lung cancer, which may play an important role in the development of lung cancer, invasion and metastasis. The expression of cystathithione- 纬 -lyase in lung cancer cells and the effect of cystathion- 纬 -lyase on proliferation and apoptosis of lung cancer cells will be helpful to understand the biological effects of cystathion- 纬 -lyase on lung cancer tumors. It may provide a certain degree of theoretical basis for the further research on cystathion-gamma lyase and lung cancer, and provide some strategies and means for the prevention and treatment of lung cancer. Therefore, the new idea of cystathithione- 纬 -lyase as a target may be used as a reference index to evaluate the early occurrence and prognosis of lung cancer.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R734.2
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