丙酮酸脱氢酶激酶4在结肠癌组织中的表达及临床意义

发布时间：2018-07-25 09:13

【摘要】：目的检测丙酮酸脱氢酶激酶4(pyruvate dehydrogenase kinase 4,PDK4)在结肠癌组织及癌旁黏膜组织中的表达情况,初步探讨其临床意义。方法应用组织芯片技术结合免疫组织化学染色方法,检测本院收治的172例结肠癌患者术后组织标本及86例癌旁肠黏膜组织中PDK4蛋白的表达,对结果及临床病理学参数进行统计学分析。结果 PDK4在172例结肠癌组织中高表达率显著高于86例癌旁肠黏膜组织(57.0%vs 26.7%,P0.01),PDK4蛋白表达高低与结肠癌的分化程度、临床分期、淋巴结转移、预后等密切相关(P0.05),且PDK4高表达组患者的生存时间显著低于低表达组患者(P0.01)。在临床分期低的结肠癌患者中,PDK高表达者预后较差(P0.01);而在临床分期高的结肠癌患者中,PDK表达高低预后差异无统计学意义。结论 PDK4蛋白在结肠癌组织中表达高于癌旁肠黏膜组织,其可能参与结肠癌的发生、发展。
[Abstract]:Objective to detect the expression of pyruvate dehydrogenase kinase 4 (PDK4) in colon cancer tissues and adjacent mucosa, and to explore its clinical significance. Methods tissue microarray technique and immunohistochemical staining were used to detect the expression of PDK4 protein in the tissues of 172 patients with colon cancer and 86 patients with adjacent intestinal mucosa. The results and clinicopathological parameters were analyzed statistically. Results the high expression rate of PDK4 in 172 cases of colon cancer was significantly higher than that in 86 cases of adjacent intestinal mucosa (57.0%vs 26.7), and the expression of PDK4 protein was significantly higher than that in the adjacent mucosa of colon cancer (P0.01) and the degree of differentiation, clinical stage and lymph node metastasis of colon cancer. Prognosis was closely related (P0.05), and the survival time of patients with high expression of PDK4 was significantly lower than that of patients with low expression (P0.01). The prognosis of patients with high expression of PDK was poor in patients with low clinical stage (P0.01), but there was no significant difference in the prognosis of PDK expression in patients with high clinical stage (P0.01). Conclusion the expression of PDK4 protein in colon cancer tissues is higher than that in adjacent mucosa tissues, which may be involved in the occurrence and development of colon cancer.
【作者单位】：第三军医大学西南医院肿瘤科;
【基金】：国家自然科学基金面上项目(81272364)~~
【分类号】：R735.35

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