阴道菌群改变与宫颈癌前病变相关性的初步研究

发布时间：2018-07-31 10:14

【摘要】：目的本实验对130例健康、HPV感染[HPV(+)]及不同级别宫颈病变(CIN)患者阴道菌群进行研究分析,揭示阴道菌群变化与宫颈病变的发生及HPV感染的关系,为阻断宫颈病变的进一步发展提供理论依据。方法收集2014-2015年大连市妇幼保健院门诊就诊的20~65岁患者130例,依据宫颈HPV检测和阴道镜下宫颈活组织检查病理诊断结果进行分组。采用QIAamp DNA mini和Blood mini试剂盒提取阴道菌群基因组DNA。利用PCR-DGGE技术对患者阴道菌群的变化进行分析,Quantity One软件进行聚类分析及凝胶测序法分析特异条带的种类。上述数据的处理和分析采用SPSS 19.0统计软件。结果在DGGE图谱中,HPV(+)组阴道菌群丰度高于HPV(-)组。CIN组阴道菌群丰度较HPV(+)组升高。测序结果显示,HPV(-)组与HPV(+)组中3条共有强度较大的条带均属于厚壁菌门,HPV(+)组的特征谱带为阴道加德纳菌(Gardnerella vaginalis)。聚类分析和主成分分析(PCA)显示,HPV(+)组患者发生了菌群失调。结论乳酸菌为阴道菌群中的优势菌;放线菌是HPV(+)的CIN患者的特殊细菌门,该菌属于阴道加德纳菌;HPV感染可引起阴道菌群稳态失调,且宫颈病变程度越重,阴道菌群失衡情况也相应加重。
[Abstract]:Objective to study and analyze the vaginal flora of 130 healthy patients with HPV () infection and (CIN) with different grades of cervical lesions, and to reveal the relationship between the changes of vaginal flora and the occurrence of cervical lesions and HPV infection. To block the further development of cervical lesions to provide a theoretical basis. Methods 130 patients aged 20 ~ 65 years old from outpatient clinic of Dalian Maternal and Child Health Hospital from 2014 to 2015 were collected and divided into groups according to cervical HPV test and pathological diagnosis of cervical biopsy under colposcopy. The genomes of vaginal flora were extracted by QIAamp DNA mini and Blood mini kit. PCR-DGGE technique was used to analyze the changes of vaginal flora in patients. The qualitative One software was used to cluster analysis and the gel sequencing method was used to analyze the species of specific bands. SPSS 19.0 was used to process and analyze the above data. Results in the DGGE map, the vaginal flora abundance of HPV (-) group was higher than that of HPV (-) group. The vaginal flora abundance of cin group was higher than that of HPV () group. The results of sequencing showed that there were three strong bands in HPV () group and HPV- group. The characteristic band of (Gardnerella vaginalis). Group was Gardnerella vaginalis. Cluster analysis and principal component analysis (PCA) showed that the patients in the HPV-group had dysbacteriosis. Conclusion Lactobacillus is the dominant bacteria in the vaginal flora, actinomycetes is a special bacterial phylum for CIN patients with HPV (), and the infection of Gardnebacterium vaginalis can cause the imbalance of the vagina flora, and the more serious the cervical lesions, the more serious the cervical lesions are. The imbalance of vaginal flora was also aggravated.
【作者单位】：大连医科大学附属大连市妇产医院暨大连市妇幼保健院妇科
【分类号】：R737.33

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