CARD9蛋白在乳腺癌组织中的表达及其临床意义

发布时间：2018-08-03 18:43

【摘要】：目的:探讨胱天蛋白酶募集域蛋白9(caspase recruitment domain containing protein 9,CARD9)在乳腺癌中的表达及其临床意义。方法:应用实时荧光定量PCR和蛋白质印迹法检测乳腺癌细胞、正常乳腺细胞、乳腺癌组织和癌旁组织中CARD9 mRNA和蛋白的表达,分析CARD9表达与乳腺癌患者临床病理特征间的关系。应用实时荧光定量PCR法检测乳腺癌组织和癌旁组织中核因子κB(nuclear factor-κB,NF-κB)信号通路相关因子p50、p65、白细胞介素1β(interlukin-1β,IL-1β)和IL-12 mRNA的表达,蛋白质印迹法检测乳腺癌组织和癌旁组织中p50、p65、抑制性NF-κB激酶α(inhibitor of NF-κB kinaseα,IKKα)和磷酸化IKKα(phosphorylated-IKKα,p-IKKα)蛋白的表达。WST-1法检测CARD9高表达对乳腺癌细胞增殖的影响。结果:乳腺癌组织中CARD9 mRNA(P0.01)和蛋白(P=0.012)表达水平显著高于癌旁组织。乳腺癌T-47D细胞(P=0.021)和MCF-7细胞(P=0.014)中CARD9 mRNA表达水平高于正常乳腺细胞Hs578Bst,乳腺癌ZR-75-1细胞中CARD9 mRNA(P=0.003)和蛋白(P=0.015)表达水平均高于正常乳腺细胞Hs578Bst。CARD9蛋白表达与肿瘤大小和雌激素受体(estrogen receptor,ER)表达有关(P值均0.05)。乳腺癌组织中p50(P0.05)和p-IKKα(P0.01)蛋白的表达水平高于癌旁组织,而且乳腺癌组织中CARD9 mRNA表达与p50(r=0.622,P0.001)、p65(r=0.392,P=0.005)、IL-1β(r=0.685,P0.001)和IL-12(r=0.556,P0.001)mRNA表达间呈正相关。CARD9高表达可促进乳腺癌细胞的增殖(P0.05)。结论:CARD9可能通过激活NF-κB信号通路而促进细胞增殖,参与乳腺癌的发生和发展。
[Abstract]:Objective: to investigate the expression and clinical significance of cystatin recruitment domain protein 9 (caspase recruitment domain containing protein 9 CARD9 in breast cancer. Methods: the expression of CARD9 mRNA and protein in breast cancer cells, normal breast cells, breast cancer tissues and adjacent tissues were detected by real-time fluorescence quantitative PCR and Western blot. The relationship between CARD9 expression and clinicopathological features of breast cancer patients was analyzed. The expression of nuclear factor- 魏 B (NF- 魏 B) signaling transduction factors p50 p65, interleukin-1 尾 (interlukin-1 尾 -IL-1 尾) and IL-12 mRNA in breast cancer and adjacent tissues were detected by real-time fluorescence quantitative PCR assay. The effects of high expression of CARD9 on the proliferation of breast cancer cells were detected by Western blot. The expression of p50 p65, inhibitory NF- 魏 B kinase 伪 (inhibitor of NF- 魏 B kinase 伪 Ike 伪 and phosphorylated IKK 伪 (phosphorylated-IKK 伪 -pIKK 伪) in breast cancer tissues and adjacent tissues were detected by WST-1 method. Results: the expression levels of CARD9 mRNA (P0.01) and protein (P0. 012) in breast cancer tissues were significantly higher than those in paracancerous tissues. The expression of CARD9 mRNA in breast cancer T-47D cells (Pn0.021) and MCF-7 cells (Pn0.014) was higher than that in normal breast cells Hs578Bst.Breast cancer ZR-75-1 cells expressed CARD9 mRNA (Pn0.003) and protein (Pn0.015) higher than those in normal breast cells, tumor size and estrogen receptor. The expression of estrogen receptor ER was related (P < 0. 05). The expression levels of p50 (P0.05) and p-IKK 伪 (P0.01) in breast cancer tissues were higher than those in adjacent tissues, and the expression of CARD9 mRNA was positively correlated with the expression of p50 (0.392p0.001) p65 (0.392P ~ (0.005) and IL-12 (r 0.556P ~ (0.001) mRNA (P0.05). Conclusion the cell proliferation may be promoted by the activation of NF- 魏 B signaling pathway, and may be involved in the occurrence and development of breast cancer.
【作者单位】：兰州大学生命科学学院细胞生物学研究所;甘肃省医学科学研究院(甘肃省肿瘤医院)转化医学研究中心;
【基金】：甘肃省卫生行业科研计划项目(编号:GSWSKY-2015-26)~~
【分类号】：R737.9

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