HSPA2在胰腺癌中的表达及其临床意义
发布时间：2018-08-07 16:06
【摘要】：目的检测并对比热休克蛋白A2(HSPA2)在胰腺癌及癌旁胰腺组织中的表达水平,分析HSPA2蛋白在胰腺癌标本中的表达水平与其临床病理参数之间的相关性。方法采用实时定量PCR、Western blot法检测并对比胰腺癌及癌旁胰腺组织中HSPA2蛋白和mRNA的表达水平,采用免疫组织化学染色方法测定胰腺癌患者组织中HSPA2蛋白的表达,并探讨其表达与临床病理特征之间的关系。结果与癌旁胰腺组织相比,胰腺癌组织中HSPA2 mRNA和蛋白表达水平均明显升高。HSPA2蛋白在胰腺癌组织中的阳性表达率为68.8%(55/80)。HSPA2蛋白表达量与胰腺癌的分化程度、血管侵犯、TNM分期差异有统计学意义(P=0.011、0.005、0.012),而与患者性别、年龄、肿块大小及位置、血清CA199水平差异无统计学意义。结论 HSPA2在胰腺癌组织中的表达明显高于与之相应的癌旁胰腺组织,且HSPA2的高表达与胰腺癌的恶性进展、侵袭和转移等事件密切相关。
[Abstract]:Objective to detect the expression level of specific heat shock protein A2 (HSPA2) in pancreatic cancer and adjacent pancreatic tissue, and to analyze the correlation between the expression level of HSPA2 protein in pancreatic cancer specimens and its clinicopathological parameters. Methods the real-time quantitative PCR, Western blot method were used to detect and compare the HSPA2 protein and M in pancreatic cancer and the pancreatic tissue adjacent to the cancer. The expression level of RNA was measured by immunohistochemical staining, and the expression of HSPA2 protein in the tissues of pancreatic cancer patients was measured and the relationship between the expression and the clinicopathological features was discussed. The results showed that the expression level of HSPA2 mRNA and protein in pancreatic cancer tissues significantly increased the positive of.HSPA2 protein in pancreatic cancer tissues. The expression rate of 68.8% (55/80).HSPA2 protein was significantly different from that of pancreatic cancer, vascular invasion and TNM staging (P=0.011,0.005,0.012), but there was no significant difference between the sex, age, the size and position of the tumor and the level of CA199 in the patients. Conclusion the expression of HSPA2 in the pancreatic cancer tissues is significantly higher than that of the same. The high expression of HSPA2 in adjacent pancreatic tissues is closely related to the malignant progression, invasion and metastasis of pancreatic cancer.
【作者单位】： 安徽医科大学附属省立医院普通外科;
【基金】：国家自然科学基金(编号:81272397) 安徽省自然科学基金面上项目(编号:1408085MKL70)
【分类号】：R735.9
本文编号：2170568
[Abstract]:Objective to detect the expression level of specific heat shock protein A2 (HSPA2) in pancreatic cancer and adjacent pancreatic tissue, and to analyze the correlation between the expression level of HSPA2 protein in pancreatic cancer specimens and its clinicopathological parameters. Methods the real-time quantitative PCR, Western blot method were used to detect and compare the HSPA2 protein and M in pancreatic cancer and the pancreatic tissue adjacent to the cancer. The expression level of RNA was measured by immunohistochemical staining, and the expression of HSPA2 protein in the tissues of pancreatic cancer patients was measured and the relationship between the expression and the clinicopathological features was discussed. The results showed that the expression level of HSPA2 mRNA and protein in pancreatic cancer tissues significantly increased the positive of.HSPA2 protein in pancreatic cancer tissues. The expression rate of 68.8% (55/80).HSPA2 protein was significantly different from that of pancreatic cancer, vascular invasion and TNM staging (P=0.011,0.005,0.012), but there was no significant difference between the sex, age, the size and position of the tumor and the level of CA199 in the patients. Conclusion the expression of HSPA2 in the pancreatic cancer tissues is significantly higher than that of the same. The high expression of HSPA2 in adjacent pancreatic tissues is closely related to the malignant progression, invasion and metastasis of pancreatic cancer.
【作者单位】： 安徽医科大学附属省立医院普通外科;
【基金】：国家自然科学基金(编号:81272397) 安徽省自然科学基金面上项目(编号:1408085MKL70)
【分类号】：R735.9
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