膀胱癌相关lncRNA及其共表达mRNA的初步筛选与功能预测
发布时间：2018-08-07 17:03
【摘要】：目的筛选出在膀胱癌组织中特异性表达的长链非编码RNA(lncRNA)及其共表达mRNA,并进行初步验证及功能预测。方法采用lncRNA v4.0芯片筛选4对膀胱癌和癌旁组织中lncRNA及其共表达mRNA的差异表达谱,通过聚类分析比较二者表达差异;采用实时荧光定量PCR(qRT-PCR)法对5个异常调节的lncRNA(RP11-359E19.2、AL928768.3、AC002519.6、RP11-79H23.3、AK021804)和4个共表达的mRNA(HRAS、VEGFA、ITGB1、DNMT3B)进行验证。同时,对lncRNA共表达的mRNA进行GO及KEGG pathway富集分析。结果差异表达的lncRNA共4 155条,其中2 045条高表达、2 110条低表达;与lncRNA共表达的mRNA共4 416条,其中2 472条高表达、1 944条低表达。|FC|≥10的lncRNA 345条,包括127条高表达和218条低表达。RP11-436F21.1和H19是上调最明显的lncRNA。|FC|≥10的共表达mRNA有75条,其中57条上调、18条下调。与癌旁组织相比,膀胱癌组织5种lncRNA中RP11-359E19.2表达上调,AL928768.3、AC002519.6、RP11-79H23.3及AK021804表达下调(P均0.05);4种共表达的mRNA中HRAS、VEGFA、ITGB1和DNMT3B表达均上调(P均0.05);qRT-PCR结果与芯片结果一致。GO分析显示,上调的共表达mRNA主要参与细胞代谢和有丝分裂的生物学过程,下调的共表达mRNAs主要参与免疫系统的刺激反应和免疫应答;KEGG pathway富集分析显示,10个通路与上调或下调的共表达mRNA有关,其中在上调的共表达mRNA中,p53信号通路和膀胱癌的富集度最高,在下调的共表达mRNA中细胞因子-因子受体相互作用富集度最高。结论成功筛选出膀胱癌特异表达的lncRNA及其共表达mRNA,这些特异表达的lncRNA及共表达mRNA在膀胱癌的发生、发展和转移中发挥重要的生物学作用,有望成为膀胱癌新的标志物。
[Abstract]:Objective to screen the long chain noncoding RNA (lncRNA) and its coexpression mRNAs specifically expressed in bladder cancer tissues, and to carry out preliminary verification and functional prediction. Methods lncRNA v4.0 microarray was used to screen the differential expression profiles of lncRNA and its coexpression mRNA in bladder cancer and adjacent tissues, and the difference between them was compared by cluster analysis. Five abnormal regulated lncRNA (RP11-359E19.2HAL928768.3) and four co-expressed mRNA (HRAS-VEGFAITGB1DNMT3B) were tested by real-time fluorescence quantitative PCR (qRT-PCR). At the same time, the mRNA co-expressed by lncRNA were analyzed by go and KEGG pathway enrichment. Results there were 4 155 differentially expressed lncRNA, 2 045 high expression and 2 110 low expression, 4 416 mRNA coexpressed with lncRNA, 2 472 high expression and 1 944 low expression, 345 lncRNA with FC 鈮，
本文编号：2170733
[Abstract]:Objective to screen the long chain noncoding RNA (lncRNA) and its coexpression mRNAs specifically expressed in bladder cancer tissues, and to carry out preliminary verification and functional prediction. Methods lncRNA v4.0 microarray was used to screen the differential expression profiles of lncRNA and its coexpression mRNA in bladder cancer and adjacent tissues, and the difference between them was compared by cluster analysis. Five abnormal regulated lncRNA (RP11-359E19.2HAL928768.3) and four co-expressed mRNA (HRAS-VEGFAITGB1DNMT3B) were tested by real-time fluorescence quantitative PCR (qRT-PCR). At the same time, the mRNA co-expressed by lncRNA were analyzed by go and KEGG pathway enrichment. Results there were 4 155 differentially expressed lncRNA, 2 045 high expression and 2 110 low expression, 4 416 mRNA coexpressed with lncRNA, 2 472 high expression and 1 944 low expression, 345 lncRNA with FC 鈮，
本文编号：2170733
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