miRNA单核苷酸多态性与宫颈癌的相关性研究

发布时间：2018-08-08 21:04

【摘要】：目的探讨miR-27a、miR-34a/c和miR-107单核苷酸多态性与宫颈癌的相关性。方法收集104例宫颈癌及186例对照,采用多重PCR技术对miR-27a,miR-34a/c和miR-107单核苷酸多态性进行分析,卡方检验分析各基因型与宫颈癌之间的相关性。结果在病例组和对照组中,miR-27a rs895819 TT、CT和CC基因型的分布为52.9%、44.1%、2.9%和59.8%、33.7%、6.5%,miR-34b/c rs4938723 TT、CT和CC基因型的分布为51.9%、35.6%、12.5%和47.3%、44.1%、8.6%,miR-107 rs2296616 TT、CT和CC基因型的分布为80.8%、19.2%、0%和81.2%、17.7%、1.1%,各位点基因型频数分布差别均无统计学意义(P0.05)。进一步分层分析发现,不同年龄的各基因型分布在对照组和病例组中差异也均无统计学意义(P0.05)。结论 miR-27a rs895819、miR-34b/c rs4938723和miR-107 rs2296616基因多态性可能与宫颈癌发生无关。
[Abstract]:Objective to investigate the correlation between single nucleotide polymorphisms of miR-27a, miR-34a/c and miR-107 and cervical cancer. Methods 104 cases of cervical cancer and 186 cases of control were collected. Multiple PCR technique was used to analyze the single nucleotide polymorphisms of miR-27a, miR-34a/c and miR-107. The correlation between the genotypes and cervical cancer was analyzed by chi square test. The results were in the case group and the case group. In the group, the distribution of miR-27a rs895819 TT, CT and CC genotypes was 52.9%, 44.1%, 2.9% and 59.8%, 33.7%, 6.5%, miR-34b/c rs4938723 TT, CT and CC genotypes of 51.9%, 35.6%, 12.5% and 47.3%, 44.1%, 8.6%, miR-107 rs2296616. There was no statistical significance (P0.05). Further stratified analysis found that the distribution of genotypes at different ages was also not statistically significant in the control group and the case group (P0.05). Conclusion miR-27a rs895819, miR-34b/c rs4938723 and miR-107 rs2296616 gene polymorphisms may not be related to the occurrence of cervical cancer.
【作者单位】：盐城卫生职业技术学院护理学院;盐城市第一人民医院;环境医学工程教育部重点实验室/东南大学公共卫生学院;
【基金】：国家自然科学基金(81472939,81673132) 江苏省高校哲学社会科学研究项目(2016SJB880119) 盐城市医学科技发展计划项目(YK2016055,YK2013060)
【分类号】：R737.33

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