Ebp1与ErbB2在胃癌中的表达与临床意义
发布时间：2018-08-10 20:02
【摘要】：目的利用免疫组织化学方法(PV9000两步法)检测ErbB2与Ebpl蛋白在胃癌组织的表达情况,分析ErbB2与Ebp1蛋白的表达与胃癌临床病理特征之间的关系及二者在胃癌组织中表达的相关性。方法收集2011年1月至2013年11月于延边大学附属医院行手术治疗后送检的胃癌组织标本132例,其中,男性97例,女性35例：汉族85例,朝鲜族47例。年龄在38岁至81岁之间,平均年龄为63.03±9.06岁,其中,年龄60岁者42例,年龄≥60岁者90例。经病理学诊断证实,所有胃癌标本的组织学类型均为腺癌,其中高、中分化腺癌49例,低分化腺癌83例。根据胃癌病理类型(Borrmann分型)将标本分为4组：1组,隆起型(I型)3例；2组,局限溃疡型(Ⅱ型).101例；3组,浸润溃疡型(Ⅲ型),26例；4组,弥漫浸润型(Ⅳ型),2例。肿瘤位于胃底部者13例、位于胃体部者38例、位于胃窦／幽门部者81例。肿瘤长径5cm者77例、≥5cm者55例。有淋巴结转移者79例,无淋巴结转移者53例；发生远处转移者14例,无远处转移者118例。肿瘤浸润深度依据第7版胃癌TNM分期标准进行判定,其中,T1/T2：2l例,T3/T4：111例(见表1)。本实验所纳入患者术前均未曾接受化疗、放疗等辅助治疗措施。采用免疫组织化学方法(PV9000两步法)分别对132例胃癌组织中ErbB2与Ebpl蛋白的表达水平进行测定。结果胃癌组织中ErbB2蛋白过表达阳性率为38.64%, Ebpl蛋白过表达阳性率为25.76%。统计分析显示,与ErbB2过表达密切相关的临床病理特征包括胃癌病理类型、分化程度、浸润深度、淋巴结转移及远处转移(p0.05),而无论性别、年龄、民族、肿瘤大小、肿瘤位置如何变化,ErbB2过表达水平都无统计学差异(P0.05)；与Ebp1过表达密切相关的临床病理特征包括胃癌病理类型、分化程度、淋巴结转移及远处转移(P0.05),而无论性别、年龄、民族、肿瘤大小、肿瘤位。置及浸润深度如何变化,Ebp1过表达水平都无统计学差异(P0.05)。等级相关检验提示ErbB2与Ebp1在胃癌组织中的表达呈正相关关系(P0.05)。结论1.ErbB2与Ebp1蛋白表达的联合检测有助于对胃癌恶性程度及侵袭、转移倾向的判断。2.ErbB2与Ebp1蛋白在胃癌组织中的表达呈正相关关系,二者可能协同促进胃癌的发生、发展,Ebpl作为胃癌ErbB2靶向治疗的辅助生物学靶点可能具有一定的意义。
[Abstract]:Objective to detect the expression of ErbB2 and Ebpl protein in gastric carcinoma by immunohistochemical method (PV9000 two-step method) and to analyze the relationship between the expression of ErbB2 and Ebp1 protein and the clinicopathological characteristics of gastric cancer and the correlation between the expression of ErbB2 and Ebp1 protein in gastric cancer tissue. Methods from January 2011 to November 2013, 132 specimens of gastric cancer were collected from the affiliated Hospital of Yanbian University, including 97 males and 35 females: 85 Han and 47 Korean. The average age was 63.03 卤9.06 years. 42 cases were aged 60 years and 90 cases were over 60 years old. The histologic types of all gastric cancer specimens were confirmed by pathology. Among them, 49 cases were well differentiated adenocarcinoma, 83 cases were poorly differentiated adenocarcinoma. According to the pathological type of gastric cancer (Borrmann classification), the specimens were divided into 4 groups: 1 group, 3 cases of bulge type (type I), 3 cases of protuberance type (type I), 3 cases of localized ulcer type (type 鈪，
本文编号：2176048
[Abstract]:Objective to detect the expression of ErbB2 and Ebpl protein in gastric carcinoma by immunohistochemical method (PV9000 two-step method) and to analyze the relationship between the expression of ErbB2 and Ebp1 protein and the clinicopathological characteristics of gastric cancer and the correlation between the expression of ErbB2 and Ebp1 protein in gastric cancer tissue. Methods from January 2011 to November 2013, 132 specimens of gastric cancer were collected from the affiliated Hospital of Yanbian University, including 97 males and 35 females: 85 Han and 47 Korean. The average age was 63.03 卤9.06 years. 42 cases were aged 60 years and 90 cases were over 60 years old. The histologic types of all gastric cancer specimens were confirmed by pathology. Among them, 49 cases were well differentiated adenocarcinoma, 83 cases were poorly differentiated adenocarcinoma. According to the pathological type of gastric cancer (Borrmann classification), the specimens were divided into 4 groups: 1 group, 3 cases of bulge type (type I), 3 cases of protuberance type (type I), 3 cases of localized ulcer type (type 鈪，
本文编号：2176048
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