探究Coronin3蛋白在大肠癌肝转移中的意义及分子机制
发布时间：2018-08-11 09:00
【摘要】：目的:Coronin3蛋白是脊椎动物七种冠状蛋白中的一种,是高度保守的,有WD40重复序列的,可调节F-肌动蛋白和Arp2/3复合物之间的平衡,进而影响细胞肌动蛋白骨架的重组、肿瘤细胞的运动的蛋白。本次实验在于探索Coronin3蛋白在结肠癌组织,癌旁组织,肝转移组织中表达情况,结合临床、病理因素进一步分析其相关性。方法:收集并整理2010年1月至2015年12月就诊于山西省肿瘤医院,经术后病理证实诊断为同时性或异时性大肠癌肝转移,且已行手术R0切除患者的临床及病理资料。除外合并多发转移行姑息性手术治疗,伴严重的内科疾病影响患者远期生存等,满足入组条件者。其中收集的临床资料包括:患者姓名、性别、年龄,合并症,原发肿瘤的位置、浸润深度、大体分型、组织类型、分化程度,阳性淋巴结数目,肝转移的数目、位置、治疗方式,是否合并多发转移,术后治疗措施,随访远期生存及死亡原因。对于符合入组标准的患者用免疫组织化学和RT—PCR分别在蛋白水平和信使RNA水平判断Coronin3蛋白水平的表达,并结合临床、病理相关资料进行分析。采用SPSS22.0统计软件包对数据进行处理,计量资料采用均数,两组间比较采用Kruskal-Wallis法进行检验,计数资料采用卡方检验;采用Graph-pad prism进行制图。结果:1.本次小样本结直肠癌同时伴肝转移的患者中,73%(22/30)的患者大于60岁,男性略多于女性,原发肿瘤部位以结肠多见70%(21/30),大体类型以溃疡型多见,并非所有的原位肿瘤均浸润组织全层,有1例肿瘤侵出浅肌层即发生远处转移,多数2.患者阳性淋巴结数目超过4枚(N2),有6例无淋巴结转移,肝转移数目以3个以下的多见,术前CEA、AFP多数未见明显增高。3.Coronin3蛋白在癌旁组织的阳性率为46%(14/30),癌组织为63%(19/30),在肝转移组织中为67%(20/30)。Coronin3蛋白在癌旁组织中的表达为2.60±1.38,在原位肿瘤中的表达为4.43±1.34,在肝转移组织中的表达为5.12±2.32。Coronin3蛋白的mRNA在癌旁组织中的表达为0.78±0.12,在原位肿瘤中的表达为1.43±0.56,在肝转移组织中的表达为1.52±0.72。相关性分析表明,Coronin3蛋白在癌旁组织中的表达明显低于原位癌组织和肝转移灶,但不能承认原位癌组织与肝转移灶间存在明显差异。4.Coronin3蛋白的表达与转移瘤的数目相关、阳性淋巴结的数目、原发灶的分化程度有关,与患者的年龄、性别无明确相关性。结论:1.结直肠癌伴肝转移可行同时手术切除患者,以结肠癌患者多见,肝脏转移数目多数小于3个,病理大体类型以溃疡型多见。2.Coronin3蛋白在癌旁组织低表达,在癌组织与肝转移灶中高表达,提示其与肿瘤的形成有相关性。3.Coronin3蛋白的表达水平,与肿瘤的转移灶、阳性淋巴结的数目、原发肿瘤的分化程度呈相关性;但是,其在癌组织与癌旁组织无明显差异。提示其与结直肠肿瘤的进展有关。
[Abstract]:Objective: the 1: Coronin3 protein is one of seven coronal proteins in vertebrates. It is highly conserved and has WD40 repeats, which can regulate the balance between F- actin and Arp2/3 complex, and then affect the recombination of actin cytoskeleton. A protein that moves tumor cells. The purpose of this study was to investigate the expression of Coronin3 protein in colon cancer, paracancerous tissues and liver metastases, and to further analyze its correlation with clinical and pathological factors. Methods: the clinical and pathological data of patients with liver metastasis from January 2010 to December 2015 in Shanxi Cancer Hospital were collected and analyzed, which were proved to be simultaneous or allogeneic liver metastases by pathology and had been resected by R0 operation. The patients with multiple metastases were treated with palliative operation, and the patients with severe internal medical diseases were affected by the long-term survival. The clinical data collected include: patient's name, sex, age, complication, location of primary tumor, depth of invasion, gross classification, histological type, degree of differentiation, number of positive lymph nodes, number and location of liver metastasis. Treatment, whether with multiple metastasis, postoperative treatment measures, follow-up long-term survival and causes of death. Immunohistochemistry and RT-PCR were used to determine the expression of Coronin3 protein at protein level and messenger RNA level in patients who met the admission criteria. The clinical and pathological data were analyzed. The data are processed by SPSS22.0 statistical software package, the mean is used to measure the data, the Kruskal-Wallis method is used to test the comparison between the two groups, the chi-square test is used to count the data, and the cartography is carried out by Graph-pad prism. The result is 1: 1. In this small sample of colorectal cancer patients with liver metastasis, 73% (22 / 30) of the patients were over 60 years old, male slightly more than female, the primary tumor location was more than 70% (21 / 30) of colon, the gross type was ulcer type, not all in situ tumors infiltrated the whole tissue layer. In one case, distant metastasis occurred in the superficial muscle layer, most of which were 2. 2%. The number of positive lymph nodes was more than 4 (N2), and no lymph node metastasis was found in 6 cases. The positive rate of coronin3 protein was 46% (14 / 30) in adjacent tissues, 63% (19 / 30) in cancer tissues, 67% (20 / 30) in liver metastases, 2.60 卤1.38 in adjacent tissues, 4.43 卤1.34 in situ tumors. The expression of mRNA with 5.12 卤2.32.Coronin3 protein was 0.78 卤0.12 in paracancerous tissues, 1.43 卤0.56 in situ tumors and 1.52 卤0.72 in liver metastases. Correlation analysis showed that the expression of Coronin3 protein in paracancerous tissues was significantly lower than that in carcinoma in situ tissues and liver metastases, but there was no obvious difference between carcinoma in situ and liver metastases. 4. The expression of Coronin3 protein was correlated with the number of metastatic tumors. The number of positive lymph nodes and the degree of differentiation of primary lesions were not correlated with age and sex. Conclusion 1. Colorectal cancer with liver metastasis can be resected at the same time. Colon cancer patients are more common, liver metastasis number is less than 3, and pathological gross type is ulcer type. 2. Coronin3 protein expression is low in adjacent tissues. The high expression of Coronin3 protein in cancer tissues and liver metastases suggests that the expression level of Coronin3 protein is correlated with tumor metastasis, the number of positive lymph nodes and the differentiation degree of primary tumor. There was no significant difference between cancer tissue and paracancerous tissue. It suggests that it is related to the progression of colorectal tumor.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.34
本文编号：2176520
[Abstract]:Objective: the 1: Coronin3 protein is one of seven coronal proteins in vertebrates. It is highly conserved and has WD40 repeats, which can regulate the balance between F- actin and Arp2/3 complex, and then affect the recombination of actin cytoskeleton. A protein that moves tumor cells. The purpose of this study was to investigate the expression of Coronin3 protein in colon cancer, paracancerous tissues and liver metastases, and to further analyze its correlation with clinical and pathological factors. Methods: the clinical and pathological data of patients with liver metastasis from January 2010 to December 2015 in Shanxi Cancer Hospital were collected and analyzed, which were proved to be simultaneous or allogeneic liver metastases by pathology and had been resected by R0 operation. The patients with multiple metastases were treated with palliative operation, and the patients with severe internal medical diseases were affected by the long-term survival. The clinical data collected include: patient's name, sex, age, complication, location of primary tumor, depth of invasion, gross classification, histological type, degree of differentiation, number of positive lymph nodes, number and location of liver metastasis. Treatment, whether with multiple metastasis, postoperative treatment measures, follow-up long-term survival and causes of death. Immunohistochemistry and RT-PCR were used to determine the expression of Coronin3 protein at protein level and messenger RNA level in patients who met the admission criteria. The clinical and pathological data were analyzed. The data are processed by SPSS22.0 statistical software package, the mean is used to measure the data, the Kruskal-Wallis method is used to test the comparison between the two groups, the chi-square test is used to count the data, and the cartography is carried out by Graph-pad prism. The result is 1: 1. In this small sample of colorectal cancer patients with liver metastasis, 73% (22 / 30) of the patients were over 60 years old, male slightly more than female, the primary tumor location was more than 70% (21 / 30) of colon, the gross type was ulcer type, not all in situ tumors infiltrated the whole tissue layer. In one case, distant metastasis occurred in the superficial muscle layer, most of which were 2. 2%. The number of positive lymph nodes was more than 4 (N2), and no lymph node metastasis was found in 6 cases. The positive rate of coronin3 protein was 46% (14 / 30) in adjacent tissues, 63% (19 / 30) in cancer tissues, 67% (20 / 30) in liver metastases, 2.60 卤1.38 in adjacent tissues, 4.43 卤1.34 in situ tumors. The expression of mRNA with 5.12 卤2.32.Coronin3 protein was 0.78 卤0.12 in paracancerous tissues, 1.43 卤0.56 in situ tumors and 1.52 卤0.72 in liver metastases. Correlation analysis showed that the expression of Coronin3 protein in paracancerous tissues was significantly lower than that in carcinoma in situ tissues and liver metastases, but there was no obvious difference between carcinoma in situ and liver metastases. 4. The expression of Coronin3 protein was correlated with the number of metastatic tumors. The number of positive lymph nodes and the degree of differentiation of primary lesions were not correlated with age and sex. Conclusion 1. Colorectal cancer with liver metastasis can be resected at the same time. Colon cancer patients are more common, liver metastasis number is less than 3, and pathological gross type is ulcer type. 2. Coronin3 protein expression is low in adjacent tissues. The high expression of Coronin3 protein in cancer tissues and liver metastases suggests that the expression level of Coronin3 protein is correlated with tumor metastasis, the number of positive lymph nodes and the differentiation degree of primary tumor. There was no significant difference between cancer tissue and paracancerous tissue. It suggests that it is related to the progression of colorectal tumor.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.34
【参考文献】
相关期刊论文 前1条
1 于萍,步宏,王华,赵高平,张景丽,周桥;免疫组化结果的图像分析与人工计数方法的对比研究[J];生物医学工程学杂志;2003年02期
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