低频超声联合载顺铂超声微泡造影剂对卵巢癌细胞增殖和凋亡的影响

发布时间：2018-08-11 15:37

【摘要】：目的探讨低频超声联合载顺铂超声微泡造影剂对卵巢癌细胞增殖和凋亡的影响及机制。方法体外培养人卵巢癌OVCAR-3细胞,随机分为对照组、超声组、顺铂组、超声联合顺铂组和超声联合顺铂微泡组。各组常规培养,顺铂组、超声联合顺铂组加入顺铂至终浓度为10μg/m L;超声联合顺铂微泡组加入载顺铂超声微泡造影剂至顺铂终浓度为10μg/m L;超声联合顺铂组、超声联合顺铂微泡组同时予低频超声处理3 min。超声组仅予低频超声处理3 min,对照组不予任何处理。培养24 h,采用CCK-8法检测各组细胞增殖能力;流式细胞术检测各组细胞凋亡率;Western blotting法检测各组Bax、Bcl-2和Caspase-3的相对表达量。结果细胞增殖能力:超声联合顺铂微泡组超声联合顺铂组顺铂组超声组对照组,组间两两比较P均0.05。细胞凋亡率:超声联合顺铂微泡组超声联合顺铂组顺铂组超声组对照组,组间两两比较P均0.05。Bax、Caspase-3的相对表达量:超声联合顺铂微泡组超声联合顺铂组顺铂组超声组对照组;Bcl-2的相对表达量:超声联合顺铂微泡组超声联合顺铂组顺铂组超声组对照组;组间两两比较P均0.05。结论低频超声联合载顺铂微泡造影剂可抑制卵巢癌细胞增殖,诱导其凋亡,其效果强于低频超声联合顺铂、单用低频超声或顺铂;其机制可能与提高细胞内Caspase-3、Bax表达,降低Bcl-2表达有关。
[Abstract]:Objective to investigate the effect and mechanism of low frequency ultrasound combined with cisplatin on proliferation and apoptosis of ovarian cancer cells. Methods Human ovarian cancer OVCAR-3 cells were cultured in vitro and randomly divided into control group, ultrasonic group, cisplatin group, ultrasound combined with cisplatin group and ultrasound combined with cisplatin microbubble group. In each group of conventional culture, cisplatin was added to the final concentration of 10 渭 g / mL in the Cisplatin group, the final concentration was 10 渭 g / mL in the ultrasound combined with cisplatin microbubble group, and the final concentration was 10 渭 g / mL in the ultrasound combined with cisplatin microbubble group, and the final concentration was 10 渭 g / mL in the ultrasound combined with cisplatin group. Ultrasound combined with cisplatin microbubble group was treated with low frequency ultrasound for 3 min. The ultrasound group was only treated with low frequency ultrasound for 3 min, while the control group was not treated with any treatment. The cell proliferation was detected by CCK-8 assay and the relative expression of Bcl-2 and Caspase-3 was detected by flow cytometry and Western blotting assay. Results Cell proliferation ability: ultrasound combined with cisplatin microbubble group ultrasound combined with cisplatin group ultrasound control group, the comparison between the two groups P 0.05. Apoptosis rate: ultrasound combined with cisplatin microbubble group ultrasound combined with cisplatin group ultrasound group control group, The relative expression of Caspase-3 was compared between two groups (P < 0.05): the relative expression of Bcl-2 in ultrasound combined with Cisplatin microbubble group and Cisplatin group: ultrasound combined with cisplatin microbubble group ultrasound combined with cisplatin group ultrasonic control group; There was a significant difference between the two groups (P < 0.05). Conclusion low frequency ultrasound combined with cisplatin microbubble contrast agent can inhibit the proliferation and induce apoptosis of ovarian cancer cells, and its effect is stronger than that of low frequency ultrasound combined with cisplatin alone, and its mechanism may be to increase the expression of Caspase-3 and Bax. The decrease of Bcl-2 expression is related to.
【作者单位】：中国医科大学附属盛京医院;
【分类号】：R737.31

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