Cytokeratin和CD133在肺腺癌患者外周血中的联合检测及临床病理分析
发布时间：2018-08-15 17:35
【摘要】：目的联合检测肺腺癌患者外周血中细胞角蛋白(cytokeratin,CK,肺腺癌循环肿瘤细胞阳性)和CD133基因(肺癌干细胞标志物)的表达,探讨二者在评估肺腺癌预后的临床价值。方法选取2014年6月至2016年6月昆明总医院心胸外科和宣威市第一人民医院心胸外科的178例未化疗的肺腺癌手术患者,中位年龄59岁,其中男性78例,女性100例。收集患者术前外周血标本10 m L,细胞块法和CK(免疫细胞化学法)用于检测CTC,PCR法用于检测CD133表达。免疫组化法检测肺腺癌组织中CK、Vimentin(Vim)、TTF-1、Ki-67、CD133的表达,并分析与外周血CK和CD133表达的关系。根据外周血CK和CD133的检测结果,将病例分为CK/CD133双阳性组和非双阳性组,统计学分析两组间临床病理因素的差异。结果 178例肺腺癌患者外周血CTC(CK阳性)的检出率为48.3%(86/178),与癌组织中Ki-67、Vim表达显著正相关(P0.05);外周血的CD133阳性表达率为66.9%(119/178),与癌组织Vim、CD133表达显著正相关(P0.05);外周血CK/CD133双阳组53例(29.8%),双阳组淋巴结转移率显著增高,肺腺癌组织学分化级别显著降低,临床分期显著提高,转移率显著增高(P0.05)。结论肺腺癌患者外周血中CK与CD133双阳性表达提示外周血存在干性特征的循环肿瘤细胞,表明预后不良,联合检测CK与CD133可用于肺腺癌患者预后监测和指导治疗。
[Abstract]:Objective to detect the expression of cytokeratin CK (positive circulating tumor cells) and CD133 gene (stem cell marker of lung cancer) in peripheral blood of patients with lung adenocarcinoma and to evaluate their clinical value in evaluating the prognosis of lung adenocarcinoma. Methods from June 2014 to June 2016, 178 patients with lung adenocarcinoma were selected from cardiothoracic surgery department of Kunming General Hospital and Cardiothoracic surgery Department of the first people's Hospital of Xuanwei City. The median age was 59 years old, including 78 males and 100 females. The peripheral blood samples of the patients were collected 10 mL before operation, and the expression of CD133 was detected by CT-PCR and CK (immunocytochemical method). Immunohistochemical method was used to detect the expression of CK-Vimentin (Vim) and Ki-67-pCD133 in lung adenocarcinoma, and the relationship between the expression of CK and CD133 in peripheral blood was analyzed. According to the detection results of CK and CD133 in peripheral blood, the patients were divided into CK/CD133 double positive group and non double positive group. The difference of clinicopathological factors between the two groups was analyzed statistically. Results the positive rate of CTC (CK expression in peripheral blood of 178 patients with lung adenocarcinoma was 48.3% (86 / 178), which was positively correlated with Ki-67Vim expression (P0.05), and the positive rate of CD133 expression in peripheral blood was 66.9% (119/ 178), which was significantly correlated with the expression of Vim-CD133 in cancer tissue (P0.05), and in peripheral blood CK/CD133 double positive group (53 cases) (P0.05). The lymph node metastasis rate in the Shuangyang group was significantly higher than that in the control group (29.8%). Histological differentiation grade, clinical stage and metastasis rate of lung adenocarcinoma decreased significantly (P0.05). Conclusion the positive expression of CK and CD133 in peripheral blood of patients with lung adenocarcinoma suggests that there are circulating tumor cells with dry characteristics in peripheral blood, indicating poor prognosis. The combined detection of CK and CD133 can be used to monitor the prognosis of patients with lung adenocarcinoma and to guide the treatment.
【作者单位】： 成都军区昆明总医院心胸外科;成都军区昆明总医院病理实验科;云南省第二人民医院急诊科;云南省宣威市第一人民医院心胸外科;
【基金】：国家教育部重点实验室开放课题(2013jszl13) 云南省应用基础青年基金(2013FD091)~~
【分类号】：R734.2
[Abstract]:Objective to detect the expression of cytokeratin CK (positive circulating tumor cells) and CD133 gene (stem cell marker of lung cancer) in peripheral blood of patients with lung adenocarcinoma and to evaluate their clinical value in evaluating the prognosis of lung adenocarcinoma. Methods from June 2014 to June 2016, 178 patients with lung adenocarcinoma were selected from cardiothoracic surgery department of Kunming General Hospital and Cardiothoracic surgery Department of the first people's Hospital of Xuanwei City. The median age was 59 years old, including 78 males and 100 females. The peripheral blood samples of the patients were collected 10 mL before operation, and the expression of CD133 was detected by CT-PCR and CK (immunocytochemical method). Immunohistochemical method was used to detect the expression of CK-Vimentin (Vim) and Ki-67-pCD133 in lung adenocarcinoma, and the relationship between the expression of CK and CD133 in peripheral blood was analyzed. According to the detection results of CK and CD133 in peripheral blood, the patients were divided into CK/CD133 double positive group and non double positive group. The difference of clinicopathological factors between the two groups was analyzed statistically. Results the positive rate of CTC (CK expression in peripheral blood of 178 patients with lung adenocarcinoma was 48.3% (86 / 178), which was positively correlated with Ki-67Vim expression (P0.05), and the positive rate of CD133 expression in peripheral blood was 66.9% (119/ 178), which was significantly correlated with the expression of Vim-CD133 in cancer tissue (P0.05), and in peripheral blood CK/CD133 double positive group (53 cases) (P0.05). The lymph node metastasis rate in the Shuangyang group was significantly higher than that in the control group (29.8%). Histological differentiation grade, clinical stage and metastasis rate of lung adenocarcinoma decreased significantly (P0.05). Conclusion the positive expression of CK and CD133 in peripheral blood of patients with lung adenocarcinoma suggests that there are circulating tumor cells with dry characteristics in peripheral blood, indicating poor prognosis. The combined detection of CK and CD133 can be used to monitor the prognosis of patients with lung adenocarcinoma and to guide the treatment.
【作者单位】： 成都军区昆明总医院心胸外科;成都军区昆明总医院病理实验科;云南省第二人民医院急诊科;云南省宣威市第一人民医院心胸外科;
【基金】：国家教育部重点实验室开放课题(2013jszl13) 云南省应用基础青年基金(2013FD091)~~
【分类号】：R734.2
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