MAT1A、MAT2A、SAMe在肝内胆管癌组织中的表达和意义

发布时间：2018-08-16 19:34

【摘要】：目的:探究MAT1A、MAT2A、SAMe在肝内胆管癌组织中的表达情况和意义;方法:搜集2012.1.26至2016.3.29于芜湖市第二人民医院及弋矶山医院行肝叶部分切除术,术后病理确诊为肝内胆管癌的60例病例资料和肝内胆管结石的86例病例资料为研究对象,病例资料包括临床病理资料及标本蜡块,分别为观察组和对照。应用组织芯片和免疫组织化学技术比较MAT1A、MAT2A、SAMe在观察组和对照组中的表达情况;分析不同物质表达之间的相关性;分析肝内胆管癌临床病理因素与物质表达的关系;并对观察组的病人进行电话随访,统计术后生存时间;结果:观察组中MAT1A、MAT2A蛋白的表达明显较对照组弱(P0.05),SAMe蛋白在观察组和对照组中的含量均较低,但观察组低于对照组,差异有统计学意义(P0.05);MAT1A、MAT2A、SAMe在肝内胆管癌组织中的表达两两之间呈正相关关系(P0.01);有胆道探查史的患者ICC组织中的MAT1A表达增多(P0.05),不同临床分期的肝内胆管癌组织中的MAT2A的表达不同(P0.05),不同分化程度的ICC组织中SAMe的含量具有差异性(P0.05);肝内胆管癌患者术后中位生存期:13月,术后1年生存率53.10%,2年生存率31.80%;结论:在肝内胆管癌的组织中MAT1A、MAT2A表达降低,SAMe的含量也较正常组织减少,以上指标的变化在肝内胆管上皮细胞的癌变过程中可能具有重要意义。
[Abstract]:Objective: to investigate the expression and significance of MAT1A- mati2An2A- SAMe in intrahepatic cholangiocarcinoma. Methods: partial liver lobectomy was performed in Wuhu second people's Hospital and Yiji Mountain Hospital from January 26 to March 29, 2016.Methods: the patients were divided into two groups: the second people's Hospital of Wuhu City and the second people's Hospital of Wuhu City. The data of 60 cases of intrahepatic cholangiocarcinoma and 86 cases of intrahepatic cholelithiasis were studied. Tissue microarray and immunohistochemical technique were used to compare the expression of MAT1Am2AMA-SAMe in observation group and control group, to analyze the correlation between the expression of different substances, to analyze the relationship between clinicopathological factors and substance expression in intrahepatic cholangiocarcinoma. The patients in the observation group were followed up by telephone to calculate the postoperative survival time. Results: the expression of MAT1Anm2A protein in the observation group was significantly lower than that in the control group (P0.05), but the content of SAMe protein in the observation group was lower than that in the control group, but it was lower in the observation group than in the control group. The difference was statistically significant (P0.05). The positive correlation was found between the expression of MAT2A in intrahepatic cholangiocarcinoma tissues (P0.01), the expression of MAT1A in ICC tissues of patients with history of exploration of biliary tract was increased (P0.05), and the expression of MAT2A in intrahepatic cholangiocarcinoma tissues with different clinical stages was significantly higher than that in patients with biliary tract exploration history (P0.01). The expression of SAMe was different (P0.05) and the content of SAMe was different in ICC tissues with different differentiation (P0.05). The median survival time of patients with intrahepatic cholangiocarcinoma was 13 months. The 1 year survival rate was 53.10%, and the 2 year survival rate was 31.80%. Conclusion: the expression of MAT1Anm2A in intrahepatic cholangiocarcinoma is lower than that in normal tissue. The changes of above indexes may play an important role in the carcinogenesis of intrahepatic bile duct epithelial cells.
【学位授予单位】：皖南医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.8

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