人外周血γδT细胞免疫治疗裸鼠皮下人肝癌细胞移植瘤的体内实验研究
发布时间：2018-08-23 15:57
【摘要】：目的:研究不同剂量的人外周血γδT细胞对裸鼠人肝癌细胞(SMMC-7721)移植瘤模型的免疫治疗作用。方法:(1)用人肝癌细胞株SMMC-7721接种BALB/c裸鼠皮下,建立肝癌裸鼠模型。(2)从健康人外周血中提取单核细胞,体外特异性扩增γδT细胞。(3)将已建立的肝癌裸鼠模型随机分为5组,阳性对照组为5-氟尿嘧啶(5-Fu),阴性对照组为生理盐水,治疗组用不同剂量的γδT细胞(1×105、5×10~5及25×10~5)分别注入裸鼠尾静脉,阳性对照组用5-Fu裸鼠腹腔注射,阴性对照组用生理盐水裸鼠尾静脉注射。观察不同剂量的γδT细胞对肿瘤的抑制效果,包括治疗前后的体重、食物摄取量及生长状况等,并与阳性对照组和阴性对照组比较肿瘤体积(TV)、相对肿瘤体积(RTV)和相对肿瘤增殖率[T/C(%)]变化。结果:不同剂量的γδT细胞对裸鼠移植瘤的生长有不同程度的抑制,RTV与生理盐水阴性对照组比较差异有统计学意义(P0.05);与5-Fu阳性对照组比较,TV增长明显低于5-Fu阳性对照组,RTV各剂量组抑制程度相似,均略高于5-Fu阳性对照组,T/C(%)各剂量组比5-Fu对照组的相对肿瘤增殖率稍低,但无显著性差异。结论:人外周血γδT细胞对肝癌裸鼠移植瘤具有显著的抑瘤作用,为建立肝癌免疫治疗新方法提供实验依据。
[Abstract]:Aim: to study the immunotherapy of human peripheral blood 纬 未 T cells in nude mice transplanted with human hepatoma cells (SMMC-7721). Methods: (1) Human hepatoma cell line SMMC-7721 was inoculated subcutaneously into BALB/c nude mice to establish hepatoma nude mice model. (2) Monocyte was extracted from healthy human peripheral blood and 纬 未 T cells were amplified in vitro. (3) the established nude mice were randomly divided into 5 groups. The positive control group was 5-fluorouracil (5-Fu), the negative control group was normal saline, the treatment group was injected into the tail vein of nude mice with different doses of 纬 未 T cells (1 脳 105 T5 脳 10 5 and 25 脳 10 5), and the positive control group was injected intraperitoneally with 5-Fu. The negative control group was injected with saline into tail vein of nude mice. The inhibitory effects of different doses of 纬 未 T cells on tumor were observed, including body weight, food intake and growth status before and after treatment. The changes of tumor volume (TV), relative to tumor volume (RTV) and tumor proliferation rate [T / C (%)] were compared with those of positive control group and negative control group. Results: different doses of 纬 未 T cells inhibited the growth of xenografts in nude mice to varying degrees (P0.05), and the growth of TV-positive cells was significantly lower than that of 5-Fu positive controls (P0.05). The inhibition degree of RTV in each dose group was similar, The relative tumor proliferation rate of each dose group was slightly lower than that of 5-Fu group, but there was no significant difference. Conclusion: human peripheral blood 纬 未 T cells have a significant inhibitory effect on hepatoma xenografts in nude mice and provide experimental evidence for the establishment of a new immunotherapy method for liver cancer.
【作者单位】： 吉林省人民医院;吉林省肿瘤防治研究所;
【基金】：吉林省科技发展计划项目(20160101197JC)资助
【分类号】：R735.7
本文编号：2199560
[Abstract]:Aim: to study the immunotherapy of human peripheral blood 纬 未 T cells in nude mice transplanted with human hepatoma cells (SMMC-7721). Methods: (1) Human hepatoma cell line SMMC-7721 was inoculated subcutaneously into BALB/c nude mice to establish hepatoma nude mice model. (2) Monocyte was extracted from healthy human peripheral blood and 纬 未 T cells were amplified in vitro. (3) the established nude mice were randomly divided into 5 groups. The positive control group was 5-fluorouracil (5-Fu), the negative control group was normal saline, the treatment group was injected into the tail vein of nude mice with different doses of 纬 未 T cells (1 脳 105 T5 脳 10 5 and 25 脳 10 5), and the positive control group was injected intraperitoneally with 5-Fu. The negative control group was injected with saline into tail vein of nude mice. The inhibitory effects of different doses of 纬 未 T cells on tumor were observed, including body weight, food intake and growth status before and after treatment. The changes of tumor volume (TV), relative to tumor volume (RTV) and tumor proliferation rate [T / C (%)] were compared with those of positive control group and negative control group. Results: different doses of 纬 未 T cells inhibited the growth of xenografts in nude mice to varying degrees (P0.05), and the growth of TV-positive cells was significantly lower than that of 5-Fu positive controls (P0.05). The inhibition degree of RTV in each dose group was similar, The relative tumor proliferation rate of each dose group was slightly lower than that of 5-Fu group, but there was no significant difference. Conclusion: human peripheral blood 纬 未 T cells have a significant inhibitory effect on hepatoma xenografts in nude mice and provide experimental evidence for the establishment of a new immunotherapy method for liver cancer.
【作者单位】： 吉林省人民医院;吉林省肿瘤防治研究所;
【基金】：吉林省科技发展计划项目(20160101197JC)资助
【分类号】：R735.7
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