二甲双胍联合奥沙利铂抑制胃癌细胞SGC-7901的增殖和诱导凋亡

发布时间：2018-08-24 09:26

【摘要】：探讨体外培养的人胃癌细胞株SGC-7901经由二甲双胍与奥沙利铂联合处理后增殖和凋亡的响应及药物作用机制。采用CCK-8增殖检测法检测药物处理下对细胞的增殖影响并计算出两种药物的IC50值。采用RT-PCR、Western blotting以及流式细胞术检测药物作用下SGC-7901细胞周期相关和凋亡相关蛋白的mRNA水平、蛋白水平和凋亡水平。实验结果显示,二甲双胍和奥沙利铂单独作用都能够抑制SGC-7901细胞的增殖,联合用药组较单独用药组对细胞的抑制率更高。二甲双胍和奥沙利铂单独作用都能够抑制SGC-7901细胞cyclin D1的mRNA和蛋白水平,药物联用后,对细胞cyclin D1的抑制表达效果更明显。与对照组相比较,二甲双胍和奥沙利铂单独作用均能显著诱导SGC-7901细胞发生凋亡(p0.01),联合用药组亡率((50.13±10.75)%)明显高于单独用药的二甲双胍组((35.06±9.17)%)和奥沙利铂组((42.59±11.98)%)。二甲双胍和奥沙利铂单独都能够抑制SGC-7901细胞中抗凋亡蛋白Bcl-2的mRNA和蛋白表达水平,并增加促凋亡蛋白Bax以及凋亡执行caspase3的mRNA和蛋白表达水平,而联合用药组中这种调控效果更加显著。二甲双胍与奥沙利铂能够抑制胃癌细胞的增殖并诱导细胞发生凋亡,可能是通过促进Bax和caspase3的表达,抑制cyclin D1和Bcl-2表达来实现的。
[Abstract]:Objective: to investigate the effect of metformin and oxaliplatin on proliferation and apoptosis of human gastric cancer cell line SGC-7901 in vitro. CCK-8 proliferation assay was used to detect the effect of drug treatment on cell proliferation and the IC50 values of two drugs were calculated. RT-PCR,Western blotting and flow cytometry were used to detect the mRNA level, protein level and apoptosis level of SGC-7901 cell cycle related protein and apoptosis-related protein. The results showed that metformin and oxaliplatin alone could inhibit the proliferation of SGC-7901 cells. Metformin and oxaliplatin alone could inhibit the mRNA and protein levels of cyclin D1 in SGC-7901 cells, and the inhibitory effect on the expression of cyclin D1 was more obvious after combined use of metformin and oxaliplatin. Compared with the control group, metformin and oxaliplatin alone could induce apoptosis of SGC-7901 cells (p0.01). The death rate in combination group (50.13 卤10.75)% was significantly higher than that in metformin group (35.06 卤9.17)% and oxaliplatin group (42.59 卤11.98)%. Metformin and oxaliplatin alone inhibited the expression of mRNA and protein of anti-apoptotic protein Bcl-2 in SGC-7901 cells, and increased the expression of apoptosis-promoting protein Bax and mRNA and protein of apoptotic caspase3. In the combined drug group, the effect of this regulation was more significant. Metformin and oxaliplatin can inhibit the proliferation of gastric cancer cells and induce apoptosis, which may be achieved by promoting the expression of Bax and caspase3 and inhibiting the expression of cyclin D1 and Bcl-2.
【作者单位】：华中科技大学附属普爱医院;
【基金】：湖北省卫生计生科研项目(WJ2015MB156)提供资助
【分类号】：R735.2

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