香乳菇多糖对Hela细胞凋亡及对RAW264.7细胞免疫活性的影响

发布时间：2018-08-25 13:24

【摘要】：为研究香乳菇多糖(LC-1)的抗肿瘤和免疫调控活性,将人的宫颈癌Hela细胞和小鼠巨噬细胞RAW264.7体外培养,分别通过CCK-8法、流式细胞术和ELISA技术检测不同浓度LC-1对Hela细胞增殖和凋亡周期的影响,对RAW264.7细胞增殖、吞噬的影响,及对RAW264.7细胞分泌NO、IL-6和TNF-α的影响。结果表明:香乳菇多糖对体外培养的Hela细胞有明显的抑制作用,且影响Hela细胞的形态及凋亡周期,当LC-1浓度为10μg/mL时,Sub峰含量可达19.4%;同时,LC-1能调控巨噬细胞的免疫活性,当LC-1浓度为10μg/mL时,巨噬细胞增值率和吞噬活性分别为67.48%和87.09%,亦能促进巨噬细胞从G0/G1期向G2期和S期转化,同时刺激巨噬细胞产生IL-6和TNF-α,且呈现出明显的剂量依赖性,但对NO生成没有明显的促进作用。综上,在体外试验中,香乳菇多糖LC-1能抑制宫颈癌Hela细胞生长,促进RAW264.7细胞增殖和吞噬活性,刺激巨噬细胞产生免疫因子。
[Abstract]:In order to study the anti-tumor and immunomodulatory activities of LC-1, human cervical cancer Hela cells and mouse macrophages RAW264.7 were cultured in vitro, respectively by CCK-8 method. The effects of different concentrations of LC-1 on the proliferation and apoptosis of Hela cells, on the proliferation and phagocytosis of RAW264.7 cells, and on the secretion of NO,IL-6 and TNF- 伪 by RAW264.7 cells were detected by flow cytometry and ELISA techniques. The results showed that the polysaccharide had obvious inhibitory effect on Hela cells cultured in vitro, and affected the morphology and apoptosis cycle of Hela cells. When the concentration of LC-1 was 10 渭 g/mL, the content of Sub peak could reach 19.4%, and LC-1 could regulate the immune activity of macrophages. When the concentration of LC-1 was 10 渭 g/mL, the proliferation rate and phagocytic activity of macrophages were 67.48% and 87.09, respectively, which could promote the transformation of macrophages from G0/G1 phase to G2 phase and S phase, and stimulate the production of IL-6 and TNF- 伪 by macrophages in a dose-dependent manner. However, there was no obvious effect on NO formation. In the in vitro experiment, LC-1 could inhibit the growth of cervical cancer Hela cells, promote the proliferation and phagocytosis of RAW264.7 cells, and stimulate macrophages to produce immune factors.
【作者单位】：西华师范大学;西华师范大学生命科学学院;西南野生动植物资源保护教育部重点实验室;
【基金】：国家自然科学基金项目(编号:31400016) 四川省生态安全与保护重点实验室开放基金资助项目(编号:ESP1408) 四川省教育厅重大培育项目(编号:16CZ0018) 南充市科技局项目(编号:16YFZJ0043)
【分类号】：R73-3

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