C12orf48在胃癌中的表达及其与临床病理特征的相关性分析

发布时间：2018-08-28 10:18

【摘要】：目的： C12orf48是新近研究发现的一个基因,在其他恶性肿瘤中已有研究,但在胃癌中的表达尚未知晓,本文初步探讨C12orf48基因在胃癌组织以及胃癌细胞系中的表达,并将其表达和胃癌患者的临床病理资料进行相关性分析,探讨其与患者临床病理特征的相关性。方法： 采用Real time-PCR和Western-blot方法检测C12orf48在胃癌细胞中的表达情况,应用免疫组织化学检查C12orf48在53例胃癌组织及对应非癌胃组织中的表达。结果： 5株胃癌细胞中,C12orf48在未分化细胞系HGC的RNA表达水平显著低于低分化胃癌细胞系AGS、KATO3、MGC和中等分化胃癌细胞系SGC7901,但C12orf48蛋白在HGC细胞中的表达水平则明显增高,而在MGC细胞中蛋白水平则较低。在胃癌标本中C12orf48阳性表达20例(37.7%),相应非癌胃组织阳性41例(77.4%),差异有统计学意义(P=0.000)。 结论： C12orf48在胃癌细胞系中表达情况存在明显差异,在胃癌组织中C12orf48的表达低于非癌胃组织,但和胃癌的浸润深度、淋巴结转移、TNM分期以及肿瘤分化程度无相关性。
[Abstract]:Objective: C12orf48 is a newly discovered gene, which has been studied in other malignant tumors, but its expression in gastric cancer has not been known. In this paper, we studied the expression of C12orf48 gene in gastric cancer tissues and gastric cancer cell lines. The correlation between the expression and clinicopathological data of gastric cancer patients was analyzed to explore the correlation between the expression and clinicopathological features of gastric cancer patients. Methods: the expression of C12orf48 in gastric cancer cells was detected by Real time-PCR and Western-blot, and the expression of C12orf48 in 53 cases of gastric cancer and corresponding non-cancerous gastric tissues was detected by immunohistochemistry. Results: the RNA expression level of C12orf48 in undifferentiated gastric cancer cell line HGC was significantly lower than that in poorly differentiated gastric cancer cell line AGS,KATO3,MGC and moderately differentiated gastric cancer cell line SGC7901, but the expression level of C12orf48 protein in HGC cells was significantly increased. However, the protein level in MGC cells was lower. The positive expression of C12orf48 was found in 20 cases (37.7%) of gastric cancer and 41 cases (77.4%) of corresponding non-cancerous gastric tissues. The difference was statistically significant (P0. 000). Conclusion: there is significant difference in the expression of C12orf48 in gastric cancer cell line, and the expression of C12orf48 in gastric cancer is lower than that in non-cancerous gastric tissue, but there is no correlation between the expression of C12orf48 and the depth of invasion, lymph node metastasis and tumor differentiation.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R735.2

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