腹腔镜下与开腹下胃癌D2根治术腹腔脱落细胞对比研究
发布时间：2018-08-28 19:16
【摘要】：目的:通过流式细胞术分别对经腹腔镜和开腹下行胃癌D2根治术患者的腹腔冲洗液中的肿瘤脱落细胞进行检测,对比分析两组肿瘤脱落细胞阳性检测率,为日后临床工作中对胃癌患者手术的方式选择以及预后评估提供临床佐证及参考,并初步探讨对胃癌腹腔肿瘤脱落细胞检测的临床意义。方法:分别收集40例经腹腔镜下行胃癌D2根治术患者的术前、中腹腔冲洗液,35例经开腹下行胃癌D2根治术患者的术前、中腹腔冲洗液。因流式细胞技术(flow cytometry,FCM)其一特性是利用DNA含量和倍体性判断细胞良恶性,利用这一特性我们将其用来对腹腔冲洗液肿瘤脱落细胞进行检测,并将测定结果显示DI≠1判定为检测阳性。术后根据分组情况将所收集的标本编号以对应相应病理检查。结果:(1)在腹腔镜组中手术前腹腔冲洗液肿瘤脱落细胞的检测阳性率为20.00%(8/40)。而开腹组术前肿瘤腹腔冲洗液中检脱落细胞的阳性率为17.14%(6/35),与腹腔镜组无显著差异(P0.05)。(2)腹腔镜组术后腹腔冲洗液肿瘤脱落细胞的检测阳性率为37.50%(15/40),与术前无显著差异。开腹组腹腔冲洗液中检测肿瘤脱落细胞的阳性率37.14%(13/35),与腹腔镜组患者无显著差异(P0.05)。结论:(1)腹腔镜下和开腹下胃癌D2根治术患者肿瘤腹腔脱落细胞检测阳性率无显著差异,由于腹腔镜相对开腹手术具有明显的微创优势,在临床工作上更适用于胃癌根治术。
[Abstract]:Objective: to detect tumor exfoliated cells in peritoneal lavage fluid of patients with gastric cancer by laparoscopy and laparotomy respectively by flow cytometry, and to compare the positive detection rate of tumor exfoliative cells between the two groups. To provide clinical evidence and reference for the selection of surgical methods and prognosis evaluation in patients with gastric cancer in the future, and to explore the clinical significance of detecting exfoliated cells in abdominal cancer of gastric cancer. Methods: 40 cases of gastric cancer D 2 radical operation under laparoscope and 35 cases of gastric cancer D 2 radical operation under laparoscopy were collected. One of the characteristics of flow cytometry (flow cytometry,FCM) is to use the content and ploidy of DNA to judge the benign and malignant cells, and we use it to detect the exfoliated cells in peritoneal lavage fluid. The results showed that DI 鈮，
本文编号：2210352
[Abstract]:Objective: to detect tumor exfoliated cells in peritoneal lavage fluid of patients with gastric cancer by laparoscopy and laparotomy respectively by flow cytometry, and to compare the positive detection rate of tumor exfoliative cells between the two groups. To provide clinical evidence and reference for the selection of surgical methods and prognosis evaluation in patients with gastric cancer in the future, and to explore the clinical significance of detecting exfoliated cells in abdominal cancer of gastric cancer. Methods: 40 cases of gastric cancer D 2 radical operation under laparoscope and 35 cases of gastric cancer D 2 radical operation under laparoscopy were collected. One of the characteristics of flow cytometry (flow cytometry,FCM) is to use the content and ploidy of DNA to judge the benign and malignant cells, and we use it to detect the exfoliated cells in peritoneal lavage fluid. The results showed that DI 鈮，
本文编号：2210352
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