上皮间质转化和肿瘤干细胞在胰腺导管腺癌不同部位
发布时间：2018-08-30 11:29
【摘要】：目的:肿瘤内部上皮间质转化和肿瘤干细胞的差异很少被研究。本研究的目的是研究上皮间质转化和肿瘤干细胞相关标志在人胰腺导管腺癌的不同部位的表达状态并探索他们和临床病理特征的关联。方法:胰腺导管腺癌瘤体被从中心到外周平均等分为3个部分,即核心、中间和外周。从20位经历手术患者的切除标本中获得胰腺导管腺癌的三个不同部位的癌组织和相对应的癌旁组织。病理图像分析的软件Image Pro Plus 6.0被用来量化免疫组化染色结果。总光密度值IOD代表肿瘤干细胞标志物CD44和上皮间质转化标志物E-cadherin和N-cadherin的表达水平。胰腺导管腺癌的肿瘤干细胞和上皮间质转化标志物的表达水平的临床意义也被分析。结果:胰腺导管腺癌组织与相应的癌旁组织中CD44,E-cadherin和N-cadherin的表达显着不同(P0.05)。然而,胰腺导管腺癌核心,中部和外围区域上皮间质转化和肿瘤干细胞标志物无显着差异(P0.05)。此外,CD44和N-cadherin的高表达,以及E-cadherin低表达与淋巴结转移和血管侵袭有关(P0.05)。CD44与N-cadherin 呈正相关(P0.05),E-cadherin 与 CD44 和 N-cadherin 呈负相关(P0.05)。结论:CD44和N-cadherin的高水平表达和E-cadherin的低水平表达和淋巴转移和血管侵犯有关联。再者,在胰腺导管腺癌,肿瘤干细胞特征和上皮间质转化程序密切相关。
[Abstract]:Objective: the difference between intraepithelial mesenchymal transformation and stem cells has rarely been studied. The purpose of this study was to investigate the expression of epithelial mesenchymal transformation and tumor stem cell related markers in different parts of human pancreatic ductal adenocarcinoma and to explore their association with clinicopathological features. Methods: pancreatic ductal adenocarcinoma was divided into three parts: core, middle and periphery. Three different tumor tissues and corresponding paracancerous tissues of pancreatic ductal adenocarcinoma were obtained from 20 surgical patients. The pathological image analysis software Image Pro Plus 6.0 was used to quantify the immunohistochemical staining results. Total optical density (IOD) represents the expression level of tumor stem cell marker CD44 and epithelial mesenchymal transformation marker E-cadherin and N-cadherin. The clinical significance of the expression of tumor stem cells and epithelial mesenchymal transformation markers in pancreatic ductal adenocarcinoma was also analyzed. Results: the expression of CD44,E-cadherin and N-cadherin in pancreatic ductal adenocarcinoma tissues was significantly different from that in corresponding paracancerous tissues (P0.05). However there was no significant difference in epithelial mesenchymal transformation and tumor stem cell markers in the core central and peripheral areas of pancreatic ductal adenocarcinoma (P0.05). In addition, the high expression of CD44 and N-cadherin, and the low expression of E-cadherin were related to lymph node metastasis and vascular invasion (P0.05). CD44 was positively correlated with N-cadherin (P0.05) E-cadherin was negatively correlated with CD44 and N-cadherin (P0.05). Conclusion the high expression of CD44 and N-cadherin is associated with the low expression of E-cadherin and lymphatic metastasis and vascular invasion. Furthermore, in pancreatic ductal adenocarcinoma, the characteristics of tumor stem cells are closely related to the epithelial interstitial transformation process.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.9
[Abstract]:Objective: the difference between intraepithelial mesenchymal transformation and stem cells has rarely been studied. The purpose of this study was to investigate the expression of epithelial mesenchymal transformation and tumor stem cell related markers in different parts of human pancreatic ductal adenocarcinoma and to explore their association with clinicopathological features. Methods: pancreatic ductal adenocarcinoma was divided into three parts: core, middle and periphery. Three different tumor tissues and corresponding paracancerous tissues of pancreatic ductal adenocarcinoma were obtained from 20 surgical patients. The pathological image analysis software Image Pro Plus 6.0 was used to quantify the immunohistochemical staining results. Total optical density (IOD) represents the expression level of tumor stem cell marker CD44 and epithelial mesenchymal transformation marker E-cadherin and N-cadherin. The clinical significance of the expression of tumor stem cells and epithelial mesenchymal transformation markers in pancreatic ductal adenocarcinoma was also analyzed. Results: the expression of CD44,E-cadherin and N-cadherin in pancreatic ductal adenocarcinoma tissues was significantly different from that in corresponding paracancerous tissues (P0.05). However there was no significant difference in epithelial mesenchymal transformation and tumor stem cell markers in the core central and peripheral areas of pancreatic ductal adenocarcinoma (P0.05). In addition, the high expression of CD44 and N-cadherin, and the low expression of E-cadherin were related to lymph node metastasis and vascular invasion (P0.05). CD44 was positively correlated with N-cadherin (P0.05) E-cadherin was negatively correlated with CD44 and N-cadherin (P0.05). Conclusion the high expression of CD44 and N-cadherin is associated with the low expression of E-cadherin and lymphatic metastasis and vascular invasion. Furthermore, in pancreatic ductal adenocarcinoma, the characteristics of tumor stem cells are closely related to the epithelial interstitial transformation process.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.9
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