GPSM2过表达对人胰腺癌细胞迁移能力的影响
发布时间：2018-09-02 05:50
【摘要】：目的:构建G蛋白信号调节蛋白2(GPSM2)稳定高表达的胰腺癌细胞株,探讨GPSM2与人胰腺癌细胞迁移能力的关系。方法:构建GPSM2基因过表达质粒(pCMV-Tag3B-GPSM2)并鉴定,将人胰腺癌MIA-PaCa-2细胞分别转染pCMV-Tag3B-GPSM2(GPSM2转染组)或p CMV-Tag3B空载体(阴性对照组),以无处理的MIA-Pa Ca-2细胞为空白对照,用RT-PCR检测各组细胞GPSM2 mRNA表达;Westernblot检测各组细胞GPSM2、β-连环蛋白(β-catenin)的表达;用Transwell实验检测各组胰腺癌细胞迁移能力。结果:成功构建了GPSM2稳定高表达的重组细胞株。与空白对照组比较,GPSM2转染组细胞GPSM2 mRNA表达量明显上调,达前者73.3倍、GPSM2、β-catenin蛋白表达量明显升高、迁移细胞计数明显增加(均P0.05)。此外,胰腺癌细胞中GPSM2与β-catenin的表达水平呈明显的正向线性关系(P0.05)。阴性对照组与空白对照组间各指标的差异均无统计学意义(均P0.05)。结论:上调胰腺癌细胞中GPSM2的表达能增加胰腺癌细胞的迁移能力,该作用可能与β-catenin蛋白表达升高有关。
[Abstract]:Aim: to construct a stable and high expression of G protein signal regulated protein 2 (GPSM2) pancreatic cancer cell line and to explore the relationship between GPSM2 and migration ability of human pancreatic cancer cells. Methods: GPSM2 gene overexpression plasmids (pCMV-Tag3B-GPSM2) were constructed and identified. MIA-PaCa-2 cells were transfected into pCMV-Tag3B-GPSM2 (GPSM2 transfection group) or pCMV-Tag3B empty vector (negative control group) respectively. Untreated MIA-Pa Ca-2 cells were used as blank control. The expression of GPSM2, 尾 -catenin (尾 -catenin) was detected by RT-PCR and the migration ability of pancreatic cancer cells was detected by Transwell assay. Results: the recombinant cell line with stable and high expression of GPSM2 was successfully constructed. Compared with the control group, the expression of GPSM2 mRNA and 尾 -catenin protein in the GPSM2 transfected group was 73.3 times higher than that in the control group, and the number of migration cells was significantly increased (P0.05). In addition, the expression of GPSM2 and 尾 -catenin in pancreatic cancer cells showed a positive linear relationship (P0.05). There was no significant difference between the negative control group and the blank control group (P0.05). Conclusion: upregulating the expression of GPSM2 in pancreatic cancer cells can increase the migration ability of pancreatic cancer cells, which may be related to the increased expression of 尾 -catenin protein.
【作者单位】： 江苏大学附属医院普通外科;
【基金】：江苏省自然科学基金资助项目(BK2012704) 江苏省博士后科研计划基金资助项目(1302096B)
【分类号】：R735.9
[Abstract]:Aim: to construct a stable and high expression of G protein signal regulated protein 2 (GPSM2) pancreatic cancer cell line and to explore the relationship between GPSM2 and migration ability of human pancreatic cancer cells. Methods: GPSM2 gene overexpression plasmids (pCMV-Tag3B-GPSM2) were constructed and identified. MIA-PaCa-2 cells were transfected into pCMV-Tag3B-GPSM2 (GPSM2 transfection group) or pCMV-Tag3B empty vector (negative control group) respectively. Untreated MIA-Pa Ca-2 cells were used as blank control. The expression of GPSM2, 尾 -catenin (尾 -catenin) was detected by RT-PCR and the migration ability of pancreatic cancer cells was detected by Transwell assay. Results: the recombinant cell line with stable and high expression of GPSM2 was successfully constructed. Compared with the control group, the expression of GPSM2 mRNA and 尾 -catenin protein in the GPSM2 transfected group was 73.3 times higher than that in the control group, and the number of migration cells was significantly increased (P0.05). In addition, the expression of GPSM2 and 尾 -catenin in pancreatic cancer cells showed a positive linear relationship (P0.05). There was no significant difference between the negative control group and the blank control group (P0.05). Conclusion: upregulating the expression of GPSM2 in pancreatic cancer cells can increase the migration ability of pancreatic cancer cells, which may be related to the increased expression of 尾 -catenin protein.
【作者单位】： 江苏大学附属医院普通外科;
【基金】：江苏省自然科学基金资助项目(BK2012704) 江苏省博士后科研计划基金资助项目(1302096B)
【分类号】：R735.9
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