SMP30对肝癌细胞行为学影响及其与糖代谢关系研究

发布时间：2018-09-03 19:11

【摘要】：糖代谢是癌症的一个典型特征,研究显示SMP30(senescence marker protein 30)是一种肝癌相关抗原,与脂代谢关系密切,为了探讨SMP30与糖代谢的变化是否相关,我们构建了稳定转染SMP30的人SK-HEP1肝癌细胞株。通过迁移实验、侵袭实验和CCK8实验对比检测了过表达SMP30组与对照组肝癌细胞的迁移、侵袭、增殖的情况;同时还检测了细胞的葡萄糖吸收、乳酸排出的情况。结果显示,与对照组相比,SMP30对SK-HEP1肝癌细胞株的迁移、侵袭有明显的抑制作用(p0.01);对细胞的增殖作用不明显(p0.05)。过表达SMP30对肝癌细胞的葡萄糖吸收和乳酸排出作用不显著(p0.05)。SMP30对肝癌细胞SK-HEP1的迁移、侵袭有一定的抑制作用,但这种作用与糖代谢关系不明显。
[Abstract]:Glucose metabolism is a typical characteristic of cancer. Studies show that SMP30 (senescence marker protein 30) is a hepatoma associated antigen, which is closely related to lipid metabolism. In order to investigate whether the change of glucose metabolism is related to SMP30, we constructed a stable SK-HEP1 hepatoma cell line transfected with SMP30. Migration, invasion and CCK8 experiments were used to detect the migration, invasion and proliferation of hepatoma cells in SMP30 group and control group, as well as glucose absorption and lactic acid excretion. The results showed that SMP30 significantly inhibited the migration and invasion of SK-HEP1 hepatoma cell line (p0.01), but had no obvious effect on cell proliferation (p0.05). Overexpression of SMP30 had no significant effect on glucose absorption and lactic acid excretion of HCC cells (p0.05). SMP30 had a certain inhibitory effect on SK-HEP1 migration and invasion of HCC cells, but this effect was not related to glucose metabolism.
【作者单位】：广西医科大学基础医学院生物化学与分子生物学教研室;
【基金】：国家自然基金(No.81460432,81572994) 广西自然基金(No.2015GXNSFDA139017) 广西壮族自治区高校科研项目(No.2013ZD011) 广西科学研究与技术开发计划项目(15104001-7) 广西南宁青秀区科学研究与技术开发计划项目资助(No.2014S03)共同资助
【分类号】：R735.7

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