miRNA-100高表达与结直肠癌淋巴结转移、TNM分期及分化程度的相关性研究
发布时间：2018-09-05 12:35
【摘要】：研究背景结直肠癌(Colorectal cancer)在我国发病率逐渐增高,因其较高的侵袭和转移特性,结直肠癌在癌症相关的死亡中占据第二位。超过30%的结直肠癌患者在自然情况下会进展成转移性结直肠癌,而转移性结直肠癌的中位生存时间小于30个月。因此,早期预测结直肠癌恶性进展以采取相应治疗措施,是提高结直肠癌患者生存期的有效方法。微小RNA(microRNA, miRNA),是一组高度保守的单链非编码RNA,它可通过靶向结合的方式特异性的结合到其下游靶基因mRNA的3'UTR区来调节其转录或降解,从而影响细胞增殖、凋亡、侵袭等生物学行为,表现为促进或抑制肿瘤发生。目的探讨微小RNA-100(miR-100)在人结直肠癌组织内表达情况与病理特征及患者预后的关系。方法采用实时定量PCR方法检测172例结直肠癌组织及癌旁组织内miR-100的表达水平,并分析其与淋巴结转移、分化程度、TNM分期及预后的关系。在体外试验中,构建miR-100高表达结直肠癌细胞系HCT-8-miR-100及对照组HCT-8-NC,观察其对结直肠癌细胞增殖、凋亡和迁移的影响。结果miR-100在结直肠癌组织中表达低于癌旁对照组织(P0.01)。结直肠癌组织中有淋巴结转移组miR-100表达直于无淋巴结转移组(P0.01)；从TNM分期Ⅰ期到Ⅳ期,miR-100表达量逐浙增加(P0.01)；从病理分化程度好到分化程度差,miR-100表达量逐渐增加(P0.01)。生存分析显示,miR-100高表达组与低表达组相比总生存率呈下降趋势,但差异无统计学意义(P=0.179)；单因素生存分析显示淋巴结转移、病理分化程度、TNM分期,是影响预后的预测因子；体外实验表明,高表达miR-100明显抑制细胞增殖,促进凋亡及迁移。结论miR-100表达升高与淋巴结转移、分化程度及TNM分期有关。MiR-100可能作为提示结直肠癌淋巴结转移及恶性进展的潜在标志物。
[Abstract]:Background the incidence of (Colorectal cancer) in colorectal cancer is increasing in China. Because of its high invasion and metastasis, colorectal cancer occupies the second place in cancer related deaths. More than 30% of patients with colorectal cancer naturally develop metastatic colorectal cancer, and the median survival time of metastatic colorectal cancer is less than 30 months. Therefore, early prediction of malignant progression of colorectal cancer and appropriate treatment measures are effective methods to improve the survival time of colorectal cancer patients. Small RNA (microRNA, miRNA), is a group of highly conserved single-strand non-coding RNA, that can specifically bind to the 3'UTR region of its downstream target gene mRNA to regulate its transcription or degradation, thus affecting cell proliferation, apoptosis, invasion and other biological behaviors. Its manifestation is to promote or suppress the occurrence of tumor. Objective to investigate the relationship between the expression of micro RNA-100 (miR-100) in human colorectal carcinoma and its pathological features and prognosis. Methods Real-time quantitative PCR was used to detect the expression of miR-100 in 172 colorectal cancer tissues and adjacent tissues, and the relationship between miR-100 expression and lymph node metastasis, differentiation and prognosis was analyzed. In vitro, the effects of miR-100 overexpression on the proliferation, apoptosis and migration of colorectal cancer cell line HCT-8-miR-100 and control group HCT-8-NC, were observed. Results the expression of miR-100 in colorectal cancer tissues was lower than that in adjacent control tissues (P0.01). The expression of miR-100 in colorectal cancer with lymph node metastasis was higher than that without lymph node metastasis (P0.01), the expression of miR-100 was increased gradually from stage 鈪，
本文编号：2224292
[Abstract]:Background the incidence of (Colorectal cancer) in colorectal cancer is increasing in China. Because of its high invasion and metastasis, colorectal cancer occupies the second place in cancer related deaths. More than 30% of patients with colorectal cancer naturally develop metastatic colorectal cancer, and the median survival time of metastatic colorectal cancer is less than 30 months. Therefore, early prediction of malignant progression of colorectal cancer and appropriate treatment measures are effective methods to improve the survival time of colorectal cancer patients. Small RNA (microRNA, miRNA), is a group of highly conserved single-strand non-coding RNA, that can specifically bind to the 3'UTR region of its downstream target gene mRNA to regulate its transcription or degradation, thus affecting cell proliferation, apoptosis, invasion and other biological behaviors. Its manifestation is to promote or suppress the occurrence of tumor. Objective to investigate the relationship between the expression of micro RNA-100 (miR-100) in human colorectal carcinoma and its pathological features and prognosis. Methods Real-time quantitative PCR was used to detect the expression of miR-100 in 172 colorectal cancer tissues and adjacent tissues, and the relationship between miR-100 expression and lymph node metastasis, differentiation and prognosis was analyzed. In vitro, the effects of miR-100 overexpression on the proliferation, apoptosis and migration of colorectal cancer cell line HCT-8-miR-100 and control group HCT-8-NC, were observed. Results the expression of miR-100 in colorectal cancer tissues was lower than that in adjacent control tissues (P0.01). The expression of miR-100 in colorectal cancer with lymph node metastasis was higher than that without lymph node metastasis (P0.01), the expression of miR-100 was increased gradually from stage 鈪，
本文编号：2224292
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